rev:      February 14, 2005

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ANTIBODIES  

(anti-Human and others as indicated)

RDI Divison of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.


PRODUCTION DATA SHEET

Monoclonal mouse anti-human IgE (4 clones availables)

CAT#: RDI-TRK1E4-4F4 or RDI-TRK1E4-5D4, or   RDI-TRK1E4-E411 or   RDI-TRK1E4-XTE4

PRICE: $375.00/mg   or   $344.00/mg 2-9     $312.00/mg 10+     Bulk quotes on request

Clone: 8E/4F4, 8E/5D4, E411, XTE4

These clones have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of a Balb/c mice immunized with IgE from myelomatous human serum.

Specificity: Clone 8E/5D4 class specific for human IgE Ce2 domain (thermostable) CAPTURE

                 Clone 8E/4F4 class specific for human IgE and directed to thermosensitive epitope localized in CE3-

                   domains. CONJUGATE (must be labeled by purchaser)

                  Clone E411 IgE Fc-region, capture or EIA

                   Clone XTE4 IgE epsilon chain, EIA

Mouse Isotype: All clones IgG2a (XTE4=mIgG1)

Application: Detection of total IgE level and IgE antibodies in ELISA.

Best pair: use MoAb 5D4 as capture and clone 4F4 as conjugate (muts conjugate yourself or inquire for custom Biotin or HRP conjugation)

MoAb 8E4F4 can be used for IgE purification by   affinity chromatography.

MoAb XTE4 can also be used in western blot

Reference: Allergol. et Immunopathol. 1978,vol 1, pp. 423-426

Allergy and Clin.Immunol.News, 1992, vol.4, 21-25

Purification: Protein A Sepharose.

Presentation: Each vial contains 1 mg in  ml (  mg/ml) PBS, pH 7.4,   containing 0.1% of sodium azide as preservative.

Note:This product is sold as an antibody preparation for research purpose only. Standard Laboratory Practices should be followed when handling this material. Contains sodium azide (0.1%) as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product FOR IN VITRO RESEARCH USE ONLY

NOTE: Recommend centrifuging the unopened vial at 500-1000 RPM for 2 minutes to concentrate antibody in bottom of vial.  If desired, you can add PBS containing 0.1% NaN3 to bring volume to 1mg in 1ml.


Material safety note: This product is sold as an antibody preparation for research purpose only. Standard Laboratory Practices should be followed when handling this material. Contains sodium azide (0.1%) as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.



FOR IN VITRO RESEARCH USE ONLY



Polyclonals to human IgE (inquire for polys to other species)

cat#RDI-A80108PB $375.00/1mg

Goat anti-human IgE:HRP 1mg

cat#RDI-A80108FB $375.00/1mg

Goat anti-human IgE:FITC 1mg

cat#RDI-A80108AB $375.00/0.1mg

Goat anti-human IgE:ALK Phos 1mg

RDI-E80108B $500.00 matched pairs for human IgE 1000 wells

-includes coating ab, HRP-conjugated secondary ab and a reference serum standard

Starter pack: contains microwell plates, buffers and TMB for use with above matched pair antibodies cat#RDI-E101PK $344.00


RDI Divison of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

Ordering terms

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