rev: 5/16/2008
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ANTIBODIES
(anti-Human and others as indicated)
RDI Division of Fitzgerald Industries Intl offers a wide line of antibodies. Since no one antibody works best for all applications (neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.
Anti-human IgG2 (see also clones 3C7 and 52G1)
Monoclonal
Product specification: mouse monoclonal anti-human IgG2
CAT#: RDI-M1326clb, new cat#: 10R-I114a PRICE: $534.75/vial
also available HRP labeled cat#RDI-M1329clb
$462.00/vial 1ml
Specificity: The monoclonal antibody was found specific for IgG2 in the
following techniques:
-haemagglutination-inhibition experiments (1,2);
-indirect systems,such as RIA, immunofluorescence and ELISA techniques (1-9).
Reactivity: The monoclonal antibody is directed against an epi tope on the Fab
fragment of IgG2 molecules (1,9). The monoclonal
antibody does not cross-react with IgG molecules of other species such as goat,
horse, rabbit and sheep.
Applications:Detection of IgG2 molecules in indirect
assays. Measurement of erythrocyte bound IgG2 antibodies. Useful as capture
antibody in IgG2 subclass ELISA (2,7,8).
Clone: CDC clone HP 6014 (1,3).
This clone has been derived from immunization of a BALB/c mouse.
Mouse isotype: The monoclonal antibody is of the mouse subclass IgG1 kappa.
Source: Ascites fluid of tumor bearing BALB/c mice.
Purification:Monoclonal antibodies were purified from
ascites using ion exchange chromatography.
Packing: Each vial contains 1 ml with antibody in 20 mM TRIS and 150 mM NaC1,pH 8.0.
Preservative:Merthiolate (0.001%).
Storage: Monoclonal antibodies should be stored below -18'C.Re peated thawing
and freezing may reduce the antibody activity and should be avoided.
Reference:
1. Jefferis,R.et al.,Immunol.Letters,31, 143 (1985).
2. Vlug,A.et al.,Prot.Biol.Fluids,36,39 (1989).
3.Reimer,C.B.et al.,Hybridoma,3,263 (1984).
4.Tyhuis,C.J.et al.,Brit.J.Haem.,77, 93 (1991).
5.Rutyhs,S.et al.,J.Immunol.Meth.,140,67 (1991).
6.Meilof,J.L.et al.,Res.Immunol.,143,711 (1992).
7.Bende,R.J.et al.,Cell Immunol.,143,310 (1992).
8.Bredius,R.C.M. et al.,Infect and Imm.,60,4838 (1992)
9.Harada,S.et al.,J.Immunol Methods,141,89 (1991).
FOR RESEARCH ONLY! NOT FOR HUMAN USE!
RDI Division of Fitzgerald Industries Intl
phone (978) 371-6446 or (800) 370-2222
fax (978) 371-2266
EMAIL:antibodies@fitzgerald-fii.com
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