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ANTIBODIES  

(anti-Human and others as indicated)

RDI Division of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.


Monoclonal Antibodies to Human IgG Subclass Antigens  (M-Series)


Product Specification: Mouse monoclonal anti-human IgG4

 

Lot#: 1271-08-01

 

CAT#: RDI-M1271clb, new cat#: 10R-I121a    PRICE: $379.50

  also available HRP labeled cat#RDI-M1331clb  $344.00/1ml

Specificity: The monoclonal antibody was found specific for IgG4   in the following techniques:

-haemagglutination inhibition experiments (1,2);

-indirect systems,such as ELISA,immunofluorescence

and RIA techniques (1-7).

 

Reactivity: The monoclonal antibody is directed against an epi tope present on the CH3 region of IgG4 (2). The monoclonal antibody does cross-react with IgG molecules of goat and sheep, but not with rabbit IgG

 

Applications:Detection of IgG4 molecules in indirect assays (1-7) Measurement of erythrocyte bound IgG4 antibodies. Useful as capture antibody in IgG4 subclass ELISA (2,4,6,7).

 

Clone: MH164-4(HP 6196) (1).

This clone has been derived from immunization of a BALB/c mouse.

 

Mouse isotype: The monoclonal antibody is of the mouse subclass IgG1 kappa.

 

Source: Ascites fluid of tumor bearing BALB/c mice.

 

Purification:Monoclonal antibodies were purified from ascites using ion exchange chromatography.

 

Packing: Each vial contains 1 ml with approximately 1.0 mg/ml antibody in 20 mM TRIS and 150 mM NaC1,pH 8.0.

 

Preservative:Merthiolate (0.001%).

 

Storage: Monoclonal antibodies should be stored below -18'C. Repeated thawing and freezing may reduce the anti body activity and should be avoided.

 

References:

1.Jefferis,R.et al.,Immunol.Letters,31,143 (1992).

2.Vlug,A.et al.,Prot.Biol.Fluids,36,39 (1989).

3.Tyhuis,C.J.et al.,Brit.J.Haem.,77,93 (1991).

4.Ruths,S.et al.,J.Immunol.Meth,140,67 (1991).

5.Meilof,J.F.et al.,Res.Immunol.,143,711 (1992).

6.Bende,R.J.et al.,Cell Immunol.,143,310 (1992).

7.Bredius,R.C.M.et al.,Infect and Imm.,60,4838(1992)

 

FOR RESEARCH ONLY! NOT FOR HUMAN USE!


RDI Division of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

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