rev: September 1, 1998

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ANTIBODIES  

(anti-Human and others as indicated)

RDI Division of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.

     $438.00/mg    $188.00/mg 100+  BULK Quotes on Request

Clone: M4C11 Presented as: Purified protein

Alternative Nomenclature: IgA study code#18

Batch:
Volume:|

Specificity: anti human IgA non IgA2m(2).

Domain: CH1

Isotype: G1

Date of Conception: May 1981

Fusion Partners of Hybridoma: Balb/c mouse spleen cell: NS1

CHARACTERIZATION:

Electrophoresis:(Ciba-Corning Universal Gel.0.05M borate buffer pH8.6.Current applied 35 mins)

a) Verification of single band and mobility identical with   reference: YES
b) Indication of concentration of monoclonal antibody >1mg/ml. YES

Haemagglutination: (Sheep RBCs coated with purified human myeloma immunoglobulin using CrC13. Ling,Bishop and Jefferis 1977.J. Immunol.Methods.15.279).

IgG coat: no reaction when tested against: Gamma 1 lambda, gamma1 kappa, gamma 2 lambda, gamma 2 lambda, gamma 2 kappa, gamma 3 lambda, gamma 3 kappa, gamma 4 labmda, gamma 4 kappa.

Other coatings:no reaction with: free lambda, free kappa, gamma 1Fc, polyclonal IgG, uLambda, uKappa free k G1Fc polyclonal IgG

positive reaction: alpha 1 lambda, alpha 2m(1)K, negative reaction: alpha 2m(2)k and delta Lambda

Additional Tests: Active in direct ELISA   Active in immunochistology

References:

Farns M.A.,Hardie D., de Lange G. and Jefferis R. (1985) Immunogenic and antigenic epitopes of human immunoglobulins X:monoclonal antibodies specific for human IgA, the IgA1 and IgA2subclasses and an nlgA2m(2) iso-allotypic epitope.Vox Sang. 48.116-121.

Biewenga J.,Faber A., de Lange G.van Leeuwen F.,ven Eede P.,Jefferis R., Hazijman J.J. and Vlug A. (1986) Monoclonal antibodies against different domains of human IgA: specificities determined by immunobloting and haemagglutination-inhibition.Mol, Immunol. 23.761-767.

Mestecky J., Hamilton R.G.,Magnusson C.G.M.,Jefferis R.,Vaerman J.P., Goodall M., de Lange G.G.,Moro I., Aucouturier P., Radl J., Cambiaso C., Silvain C.,Preud'homme J.L.,Kusama K., Carlone G.M., Biewenga J., Kobayashi K., Skvaril F. and Reimer C. (1996) Evaluation of monoclonal antibodies with specificity for human IgA, IgA subclasses and allotypes and secretory component. J.Immunol. Methods. 193.103-148.

 $438.00/mg 

Clone: M4D8

Presented as: Purified protein.

Alternative Nomenclature: IgA study code #17

Batch:
Volume:

Specificity: anti human IgA subclass 1.

Domain: CH2

Isotype: G1

Date of Conception: May 1981

Fusion Partners of Hybridoma: Balb/c mouse spleen cell: NS1

CHARACTERIZATION:

Electrophoresis: (Ciba-Corning Universal Gel. 0.05M borate buffer pH8.6.Current applied 35 mins).

a) Verification of single band and mobility identical with   reference: YES

b) Indication of concentration of monoclonal antibody > 1mg/ml. YES

Haemagglutination: (Sheep RBCs coated with purified human myeloma immunoglobulin using CrC13. Ling,Bishop and Jefferis 1977 J. Immunol.Methods. 15.279)

IgG coat: no reaction when tested against: Gamma 1 lambda, gamma1 kappa, gamma 2 lambda, gamma 2 lambda, gamma 2 kappa, gamma 3 lambda, gamma 3 kappa, gamma 4 labmda, gamma 4 kappa.

Other coatings:no reaction with: free lambda, free kappa, gamma 1Fc, polyclonal IgG, uLambda, uKappa free k G1Fc polyclonal IgG

positive reaction: alpha 1 lambda

negative reaction: alpha 2m(2)k and delta Lambda

Additional tests: Active in direct ELISA

Active in immunohistology

References:

Farris M.A.,Hardie D., de Lange G. and Jefferis R.(1985) Immunogenic and antigenic epitopes of human immunoglobulins X:monoclonal antibodies specific for human IgA, the IgA1 and IgA2 subclasses and an nlgA2m(2) iso-allotypic epitope.Vox Sang. 48, 116-121.

Biewenga J., Faber A., de Lange G. van Leeuwen F., ven Eede P.,Jefferis R., Haaijman J.J.,and Vlug A. (1986) monoclonal antibodies against different domains of human IgA: specificities determined by immunoblotting and haemagglutination-inhibition.Mole. Immunol. 23.761-767.

Mestecky J., Hamilton R.G.,Magnusson C.G.M.,Jefferis R.,Vaerman J.P., Goodall M., de Lange G.G., Moro I., Aucouturier P.,Padl J.,Cambiaso C.,Silvain C.,Preud'homme J.L.,Kusama K.,Carlone G.M.,Biewenga J., Kobayashi K.,Skvaril F. and Reimer C. (1996) Evaluation of monoclonal antibodies with specificity for human IgA,IgA subclasses and allotypes and secretory component.J.Immunol.Methods. 193,103-148.

RDI Division of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

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