rev:  September 24, 2003

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ANTIBODIES  

(anti-Human and others as indicated)

RDI Divison of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.


Mouse ANTI-HUMAN J CHAIN

PRODUCT CODE :RDI-CBL116  $375.00/vial of 200ug

CLONE: M19/9J

ISOTYPE: IgG1

SOURCE: Mouse ascitic fluid

PURIFICATION METHOD: Ion exchange chromatography following ammonium sulphate precipitation.

SPECIFICITY: Human J-chain as defined by ELISA assay using purified human immunoglobulin fragments as solid phase target antigen.

APPLICATIONS:

*Identification of surface immunoglobulins on B lymphocytes by indirect immunofluorescence staining of cell suspensions or immunoperoxidase staining of tissue sections

* Leukaemia and lymphoma phenotyping

* Clonality determination of B cell malignancies


N.B. Staining of cryostat sections is best achieved by fixation in methanol prior to application of antibody.


PRESENTATION: Each monoclonal is delivered in a sealed vial at a concentration of 2 vials of 100µg/1ml in phosphate buffered saline containing 10mM sodium azide and 1mg/ml of bovine serum albumin. We recommend that each laboratory determine an optimum working titre for use in its particular application.


STORAGE: Purified monoclonal antibodies are generally stable for long periods at 4oC. However repeated warming to room temperature and re-cooling may result in a loss of activity and hence effective working titre. It is recommended that monoclonal antibodies are aliquotted upon receipt and then stored at -20oC. After thawing they should then be used as a stock solution for up to 60 days. Dilute working solutions are best prepared on the day of use.


For research use only. Not supplied for use in human diagnostic or therapeutic procedures

Bulk milligram sizes quoted upon request (available with or without BSA or preservative)


RDI Divison of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

Ordering terms

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