rev:   September 16, 2004

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  ANTIGENS/Pure Proteins 

(for in vitro research use only)

RDI Division of Fitzgerald Industries Intl  offers a wide line of  antigens for use in histochemistry, ELISA, and further manufacturing use. Please inquire for other antigens or antibodies to use with these products. Please inquire for Bulk quantities-significant discounts available!

cat# RDI-PR3X-AG      100ug $500.00

RDI-PR3X-AGX1         10 X 100ug $3125.00

RDI-PR3X-AG1            1 X 1mg $3250.00

RDI-PR3X-AG10          10mg $25000.00

DESCRIPTION. Highly purified by chromatography. Free of elastase and cathepsin G. Prepared from 0.03 M Citric acid pH 3.5 + 1 M NaCl and <0.1% Triton® X100 ,lyophilization after dialysis vs. 1mM Citric acid pH 3.5 + 0.005% Triton(tm) X 100. The dried product contains approximately 10% PR3 protein and 90% solids. Re-dissolve with H2O to 1 mg/ml.

SOURCE. Leucocytes of Purulent Human Sputum.

MW: SDS-PAGE indicates MW = 29,000.

Purity > 95% by native electrophoresis at pH 4.3 (4) yielding predominately 2 bands that migrate one-half the distance in comparison to Cathepsin G. Elastase, Lysozyme and Myeloperoxidase are also compared. A similar electrophoretic pattern was first described by Baggiolini et.al. (2) for the three proteases.

Storage: Store frozen at -20°. Re-dissolve with H2O to 1 mg/ml. After reconstitution,may be freeze-thawed up to 5 times (avoid frequent freeze thaw cycles, recommend aliquoting and store -20 DEG C).

Activity Test: 35 - 45 units per mg of protein. One unit will hydrolyze 1mMole of Boc-Ala-Ala-Nva-SBzl per minute at pH 7.5 at 25°C. The assay buffer-substrate (2 ml) is 0.1 M MOPS pH 7.5 containing 0.5 M NaCl, 10% DMSO, 1 mM substrate and 0.1 mM 5,5'-dithiobis-(2-nitrobenzoicacid). Use 0.2 to 0.5 ug of enzyme. Read increase in absorbance at 405 nm. The 1.0 mM extinction coefficient is 13.1 in a 10 mM cell.The kinetics of PR3 hydrolysis of thiolbenzyl substrates is described by Kam et. al. (1)

For Research or Further Manufacturing Use Only

RDI Division of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

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