rev: October 14, 2004
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(for in vitro research use only)
RDI Divison of Fitzgerald Industries Intl offers a wide line of antigens for use in histochemistry, ELISA, and further manufacturing use. Please inquire for other antigens or antibodies to use with these products. Please inquire for Bulk quantities-significant discounts available!
DATA SHEET:Human ALPHAFETOPROTEIN (98%)
see also alphfetoprotein matching antibodies-
CAT#: RDI-TRK8F8 $562.00/0.5mg $1000.00/1.0mg
Source: Human . Blood sample from the tissue donor was tested and found negative for HBsAg, HIV-1 ,HIV-2 and HCV antibodies.
Purification: Immunoaffinity chromatography, biospecific chromatography
Presentation: Lyophilized from 0.1M PBS pH 7.2 with 2.5% sucrose and 0.1% NaN3. It is recommended that the product should be reconstituted in distilled water.
Analysis: Purity more 99% according to SDS-PAGE Does not contain albumin, antitrypisin or transferrin
Immunological activity confirmed by reaction with monoclonals which are specific to human alphafetoprotein.
Storage: At -20'C in lyophilized form. After reconstitution the solution should be aliquoted and stored at- 20'C. Avoid frequent freeze thaw cycles.
For In Vitro Research Use Only-Not for Use in or on human or animals-not for use in diagnostics
see also monoclonals against alphafetoprotein and pure alpha fetoprotein receptor
Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics . All starting material were tested and found negative for HBsAg and HIV-1 ,HIV-2 and HCV antibodies by an approved method. However, all material should be handled at Biosafety Level II conditions and treated as if capable of transmitting disease. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.
CAT#: RDI-TRK4F16-5H7, or RDI-TRK4F16-4A3
-other clones on special request only
PRICE: $375.00/mg or $344.00/mg 2-9 $312.00/mg 10+
Significant Bulk Discounts-please inquire
Clone: 5H7, 4A3
These clones have been derived from hybridization of SP2/0 myeloma cells
with spleen cells of a Balb/c mice immunized with purified human
Specificity: MoAb do not cross-react with human albumin.
Mouse isotype: IgG2a for clone 4A3
IgG1 for clone 5H7
MoAb 4A3 and 5H7 can be used in two-site immunoassay for detection of human a-fetoprotein.In sandwich we recommend to use MoAb 4A3 for coating and MoAb 5H7 for labeling. BEST PAIR
MoAb 7H8 can be used only for affinity purification of human a-fetoprotein.
Purification: Purified by chromatography on protein A Sepharose.
Purity is tested by electrophoresis.
Presentation: Each vial contains 1mg antibodies in 0.1ml (10.5mg/ml) PBS, pH 7.4,containing 0.1% of sodium azide as preservative. Store at +4C. SEE VIAl LABELS
Note: This product is sold as an antibody preparation for research purpose only. Standard Laboratory Practices should be followed when handling this material. Contains sodium azide (0.1%) as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product. FOR IN VITRO RESEARCH USE ONLY
NOTE: Recommend centrifuging the unopened vial at 500-1000 RPMfor 2 minutes to concentrate antibody in bottom of vial. If desired, you can add 0.9ml PBS containing 0.1% NaN3 to bring volume to 1mg in 1ml.
FOR IN VITRO RESEARCH USE ONLY. NOT FOR USE IN OR ON HUMANS OR ANIMALS-NOT FOR DIAGNOSTICS. Not responsible for any Patent infringements with use or derivation of these products. FOR IN VITRO RESEARCH USE ONLY
RDI Divison of Fitzgerald Industries Intl
34 Junction Square Drive
Concord MA 01742-3049
phone (978) 371-6446 or (800) 370-2222
fax (978) 371-2266