rev: September 1, 1999

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  ANTIGENS/Pure Proteins 

(for in vitro research use only)

Research Diagnostics Inc  offers a wide line of  antigens for use in histochemistry, ELISA, and further manufacturing use. Please inquire for other antigens or antibodies to use with these products. Please inquire for Bulk quantities-significant discounts available!

DATA SHEET:   Human Thyroid Peroxidase (RECOMBINANT)

see also TPO antibodies  for more information and matching antibodies-

see also native/natural TPO

-Human thyroid peroxidase is the primary enzyme involved in thyroid hormone synthesis. The immunological measurements of TPO and specific antibodies directed against TPO have been of great value in the screening and monitoring of Hashimoto's thyroiditis and other thyroid diseases.

Catalog#: RDI-TPO-REC-AG


Package Size:   50 micrograms    $ 300.00

                          1 milligram     $2500.00

                          2-4mg             $2100/mg

                          5-9mg             $2000.00/mg

                        10-24mg          $1900.00/mg  

                       25-50mg           $1750.00/mg      Larger on quotation



Batch# TPOREC/904  (see certificate of analysis with each shipment for any batch specific information)

expiration: August 2002

Package Size: 50 micrograms $300.00    1 milligram $2500.00

Source: Recombinant. Expressed by recombiannt baculovirus (Autographa californica nuclear polyhedrosis virus;AcNPV) infection of Spodoptera frugiperda Sf9 insect cells. expressed with a -6xHis tag

Construct: truncated cDna coding for the extracellular part of human TPO. Transmembrane and intracytolplasmic parts of the molecule are removed and replaced by a hexa-histidine purification tag at the C terminus. After cleavage of the secretory leader presequence, the mature recombinnat TPO has a length of 834 amino acids.

Purity: >95% by SDS-Page analysis, Densitometric analysis. Probed with monoclonal anti-6-His tag.

Immunreactivity: Elisa standard test, checker board analysis of positive/negative sera panels including international referece serum NIBSC 66/387)

MW: apparent=101kDa (calculated 92,872)

Isoelectric point: pH 6.2 (calculated)

Storage: -70 DEG C. At first thaw, tighten cap, centrifuge 1 minute 1000 rpm to concentrate material in vial. Store upright. Avoid frequent warming cycles. Recommend making working aliquots and store at -70 DEG C. Use of reducing sulfhydryl reagents and denaturing detergents will result in destruction of the conformational epitope.

Dilution buffers: recommend ion strength approx 150mM, neutral to slightly alkaline pH and 20% glycerol as cryoprotective agent.

Elisa: approx coating concentration 0.15-0.4ug/ml (titrate for each use)

Precautions: For In vitro research or further manufacturing use only.

It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.

Also available, natural TPO and anti-human TPO antibodies for ELISA and immunoaffinity purification.

Research Diagnostics Inc

Pleasant Hill Road

Flanders NJ 07836

phone (800) 631-9384 or (973) 584-7093

fax     (973) 584-0210

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