rev:May 27, 1997
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BIOBOND (tm) & BIOMOUNT(tm)
cat#RDI-BB20 $112.00/20ml vial $94.00/vial 3 or more
For light microscopy and electron microscopy the choice of specimen preparation si critical for the preservation of antigens in the sample. Of greatest importance in the preparation schedules are the specimen fixation and embedding. this protocol must satisfy the requirements for preservation of structural integrity and antigenicity.
Having prepared the tissue specimen for immunolabelling, it is then imperative
to perform the incubations with a protocol designed to maximize the
specific signal and minimize the background. Some incubation conditions may
cause tissue sections to be removed from the glass slide. Typical tissue
section adhesives such as poly-L-lysine, Elmer's glue, chrome alum, etc are
not suitable for use with immunogold labelling because of the increased
background caused by attraction of gold particles to the adhesive on the
In addition the surface of glass slides is uneven and is activated by the silicon tetrahedral structure. this provides active sites for absorption of proteins or reactions with chemicals and reagents. It is therefore important to minimize this possibility by coating the surface with a material that is of low reactivity towards reagents.
A new special slide coating solution, BIOBOND (tm) is available which produces a very strong adhesion between the glass and the tissue section for subsequent incubation. Biobond (tm) coats the slide with a protective layer to minimize interaction of charged glass surface with reagent. This is also of particular importance for reproducibility of results because of the variations that occur between glass slides obtained from different sources in different countries. It is particularly effective for use with severe incubating conditions such as those used in in situ hybridization. Biobond (tm) is suitable for all kinds of tissue specimens including paraffin wax or resin sections, cell smear, cytospins or cryostat sections. Biobond (tm) is supplied in 20ml unit volumes, sufficient to coat at least 1000 slides.
Simple Coating Procedure:
1) Make a 2% solution of Biobond (tm) is acetone (eg 2ml in 100ml acetone).
Use fresh solution each time.
2) Place slides in a plastic or metal holder and immerse in Biobond solutions for 4 minutes.
3) Rinse the slides in clean water for 5 minutes
4) Air Dry
-The slides are then ready for mounting sections. They may be stored indefinitely.
BIOMOUNT (tm) TISSUE SECTION MOUNTING MEDIUM
cat#RDI-BM100 $94.00/100ml vial $62.00/vial 3 or more
Biomount (tm) reduces fading of immunogold/silver signals in sections on glass slides. It is suitable for both resin and wax embedded tissue sections.
Some mounting media oxidize rapidly on exposure to air, forming carboxyl
groups. This may be especially so when sections have been cleared in a solution
containing aldehyde groups. It is sometimes observed that the immunogold/silver
stain fades after a few weeks or even in a shorter time, from sections that
have been mounted with these media under cover slips. The silver is still
present but has formed translucent silver carboxylate salts. Visibility can
be retrieved by removing the cover slip and washing in xylene (xylol) and
then immersing the slide in photographic developer, but this is a tedious
procedure. Biomount (tm) prevents this fading because of its low oxidizing
Biomount (tm) is miscible with xylene and may be applied to sections on slides in the normal manner following dehydration and immersion in xylene. Labelling will retain its intensity and contrast following the use of Biomount (tm). Sections that have been counterstained with normal stains will also retain their color intensity.
RDI Divison of researchd Industries Intl
San Jose, 95123 CA Snell ave 658
or (800) 370-2222