rev: July 8, 1998

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Sample Kit Protocol: (see actual insert with kit for batch specific information):


RAT  INTERFERON GAMMA ELISA Kit


CAT# RDI-PB43500   96 wells     PRICE: $438.00   $406.00/kit 3 or more



Storage Conditions: Keep at 4'C for greatest stability.

Sensitivity: <25 pg/ml (<0.25 units/ml). Assay range is between 0 and 2000 pg/ml.


Speed:Incubation time, 3 hours 15 minutes.


Specificity: Rat Ifn-g.


Specifications: This kit quantitates rat interferon gamma using a sandwich immunoassay. (1,2) The kit is based on an ELISA with streptavidin conjugated to horseradish peroxidase (HRP). Tetramethylbenzidine (TMB) is the substrate. All reagents are supplied One pre-coated microtiter strip plate (96 wells) is included.


Since there is no international reference standard for rat interferon gamms, pg/unit values can only be approximated.(3) A typical standard curve for each lot and a microtiter plate worksheet are included with the procedure.


QUALITY CONTROL: Standard written procedures are followed in the preparation of all ELISA kits. Each group of ELISA kits is assigned a lot number. Sample kits from each lot are retained for periodic evaluation. Kits are guaranteed for six months from the date of shipment.


1. Staehelin, T., Stahli, C., Hobbs, D.S., and Pestka, S.(1981) "A Rapid Quantitative Assay of High Sensitivity for Human Leu kocyte Interferon with Monoclonal Antibodies," in Methods in Enzymology, Vol. 79 (S.Pestka,ed.), Academic Press, New York, 589-595.

2. Kelder, B.,Rashidbaigi, A., and Pestka,S. (1986) "A Sandwich Radioimmunoassay for Human IFN-a,"in Methods in Enzymology,Vol 119 (S.Pestka,ed.), Academic Press, New York, 582-587.

3. Schellekens, H., DeWilde, G.A., Weimar, W. (1980) J. Gen.Virol Vol. 46, 243.


THIS PRODUCT IS FOR RESEARCH USE ONLY. IT IS NOT FOR HUMAN USE.


For In Vitro Research Use Only-Not for use in Diagnostics


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RDI Divison of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax      (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

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