rev: July 8, 1998

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Sample Kit Protocol: (see actual insert with kit for batch specific information):


MURINE INTERFERON GAMMA ELISA Kit


CAT# RDI-PB42500   96 wells    PRICE: $438.00   $406.00/kit 3 or more


Storage Conditions: Keep at 4'C for greatest stability.


Sensitivity: <25 pg/ml (<0.25 units/ml). Assay range is between 0 and 500 pg/ml.


Speed: Incubation time, 3 hours 15 minutes.


Specificity: Mouse Ifn-g.


Specifications: This kit quantitates mouse interferon gamma using a sandwich immunoassay. (1,2) The kit is based on an ELISA with streptavidin conjugated to horseradish peroxidase (HRP). Tetramethylbenzidine (TMB) is the substrate. All reagents are supplied. One pre-coated microtiter strip plate (96 wells) is included


The assay is based on the international standard for mouse interferon gamma (Mouse Ifn-g) provided by the National Institutes of Health.(3) A typical standard curve for each lot and a microtiter plate worksheet are included with the procedure.

QUALITY CONTROL: Standard written procedures are followed in the preparation of all ELISA kits. Each group of ELISA kits is as- signed a lot number. Sample kits from each lot are retained for periodic evaluation. Kits are guaranteed for six months from the date of shipment.

1. Staehelin,T., Stahli, C., Hobbs, D.S., and Pestka,S.(1981)"A   Rapid Quantitative Assay of High Sensitivity for Human Leuko cyte Interferon with Monoclonal Antibodies,"in Methods in En zymology, Vol. 79 (S. Pestka,ed.), Academic Press, New York, 589-595.

2. Kelder, B., Rashidbaigi, A.,and Pestka, S. (1986) "A Sandwich Radioimmunoassay for Human IFN-a," in Methods in Enzymology, Vol. 119 (S. Pestka, ed.), Academic Press, New York, 582-587.

3. Mouse Ifn-g international reference standard provided by the NIH, reference no. Gg02-901-533. Pestka, S. (1986) "Interferon Standards and General Abbreviations," in Methods of Enzymology Vol. 119 (S. Pestka,ed.), Academic Press, New York 14-23.


THIS PRODUCT IS FOR RESERCH USE ONLY. IT IS NOT FOR HUMAN USE.

For In Vitro Research Use Only-Not for use in Diagnostics


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RDI Divison of Fitzgerald Industries Intl

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Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax      (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

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