rev:    March 3, 2005

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Sample Kit Information: (see actual insert with kit for batch specific information):


MouseINTERFERON BETA ELISA Kit

# RDI-PB42400    96 wells     PRICE: $688.00


a) Range: 15.6-1,000pg/ml

b) Incubation time: 3hr, 15min

c) Specificity: Mouse IFN-Beta, no cross reactivity with human IFN-Alpha, human IFN-gamma, human IFN-Beta, mouse IFN-alpha, mIFNG, rat IFNA, B or gamma, feline IFN-alpha or pig IFN-alpha

d) The kit quantitates MOUSE interferon beta in media using a sandwich immunoassay. The kits is based on an ELISA with tetramethylbenzidine (TMB) is the substrate.  Samples in mouse serum and tissue culture media have been detecetd successfully in this assay format.

All reagents are supplied. One precoated micrototer strip plate (96 wells) is included

e) The assay is based on the international reference standard for human intterferon beta (IFN-beta) provided by the NIH.

Material supplied

1) One 96 well microtiter plate to which capture antibody is bound

2) Four plate sealers:

     -10X wash solution concentrate (50ml)

     -mouse Interferon Beta solution (50ul)

     -sample diluent    (30ml)

    -antibody concentrate 50ul (50ul)

    -HRP conjugate concentrate (50ul)

    -Conjugate Diluent (30ml)

    -TMB substrate  (15ml)

    -Stop Solution (15ml)
For In Vitro Research Use Only-Not for use in Diagnostics


Basic Protocol:

Add 100ul Blank/standard/sample to well, incubate 60 minutes RT, wash 3X

Add 100ul 2nd ab, incubate 60 minutes RT, wash 3X

Add 100ul HRP conjugate, incubate 60 minutes RT, wash 3X

Add 100ul TMB, incubate 15 minutes,

Add 100ul stop reagent

Read at 450nm.



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RDI Divison of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax      (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

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