rev:  March 28, 2005

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Sample Kit Information: (see actual insert with kit for batch specific information):


Human INTERFERON ALPHA ELISA Kit

CAT# RDI-PB41100   96 wells     PRICE: $531.00   $500.00/kit 3-19 (not for serum samples)

CAT# RDI-PB41105   96 wells     Price :   $594.00    $562.00/kit  5+   (not for serum samples)

CAT# RDI-PB41110   96 wells     Price    $594.00     $562.00/kit  5+  (For Human Serum Samples)

cat#    RDI-PB42100   96 wells     Price:   $594.00     $690.00/kit  5+  (New MOUSE SPECIFIC)

-inquire for bulk pricing on 11 or more kits  

Also Available, Bulk Pack Kits  please inquire for pricing


Most human alpha interferon assays have been created solely for the purpose of detecting interferon alpha A (Hu-IFN-alpha 2 a) or the closely related interferon alpha 2(Hu-IFN-alpha2b). These kits may also detect a few of the other alpha species, but that is typically unknown. To address the need to detect all of the human alpha interferons, cat#RDI-PB41105 has been created.

Below are 2 separate kits (the cat#RDI-PB41105 detects all of the human alpha interferons except possible alpha F).

                                                                    detection sensitivity
Interferon Species Gene cat#RDI-PB41100 cat#RDI-PB41105
Human IFN ALPHA A IFNA2 ++ ++
Human IFN Alpha 2 IFNA2 ++ ++
Human IFN ALPHA A/D - ++ ++
Human IFN Alpha B2 IFNA8 -- ++
Human IFN Alpha C IFNA10 + ++
Human IFN Alpha D IFNA1 ++ ++
Human IFN Alpha F IFNA21 -- --
Human IFN Alpha G IFNA5 + ++
Human IFN Alpha H IFNA14 -- ++
Human IFN Alpha I IFNA17 -- ++
Human IFN Alpha J IFNA7 + ++
Human IFN Alpha K IFNA6 ++ ++
Human IFN Alpha 4b IFNA4
Human IFN Alpha WA IFNA16
Human IFN Beta - -- --
Human IFN gamma - -- --
Human IFN Omega - -- --
Mouse IFN Alpha A - -- --
Mouse IFN Beta - -- --
Mouse IFN gamma - -- --
Rat IFN Alpha - -- --
Rat IFN Beta - -- --
Rat IFN gamma - -- --
Bovine IFN Tau - -- --

++ = very good, + = moderate, --  =  not al all

Alpha A=Alpha 2A,    Alpha 2=Alpha 2b (nomenclature)


HUMAN INTERFERON ALPHA (Hu-IFN-a a) ELISA KIT

Product Number: cat#RDI-PB41100

Size: single-plate kit

Lot Number:

Quantity Shipped:

Date Shipped:

Storage Conditions: Keep at 4EC for greatest stability. Range: 10-500 pg/ml for the high sensitivity protocol. 40-5000 pg/ml for the extended range protocol.

Speed: Incubation time, 3 hours 15 minutes.

Specificity: Human IFN-aA, a2, aA/D, aD, aK, and a4b. No cross-reactivity with human IFN-g or human IFN-b. No cross-reactivity with mouse or rat IFN-a, IFN-b, or IFN-g.

Sample Type: Natural or recombinant human IFN-a in solution or tissue culture media (containing FBS).

Sample Size: 100 ul

Specifications: This kit quantitates human interferon alpha using a sandwich immunoassay.1,2 The kit is based on an

ELISA with anti-secondary antibody conjugated to horseradish peroxidase (HRP). Tetramethylbenzidine (TMB) is the substrate. All reagents are supplied. One pre-coated, microtiter breakaway strip plate (96 wells) is included. The specific activity of the interferon used as the standard in the kit is based on the international standard for human interferon alpha (Hu-IFN-a) provided by the National Institutes of Health.3 A typical standard curve for each lot and a microtiter plate worksheet are included with the procedure. Please note that the concentrations of the detecting antibody and the HRP differ from lot to lot. The differences are the result of calibrating each kit for optimal sensitivity. Quality Control: Standard written procedures are followed in the preparation of all ELISA kits. Each group of ELISA kits is assigned a lot number. Sample kits from each lot are retained for periodic evaluation. Kits are guaranteed for six months from the date of production.

1. Staehelin, T., St�hli, C., Hobbs, D.S., and Pestka, S. (1981) "A Rapid Quantitative Assay of High Sensitivity for Human Leukocyte Interferon with Monoclonal Antibodies," in Methods in Enzymology, Vol. 79 (S. Pestka, ed.), Academic Press, New York, 589-595.

2. Kelder, B., Rashidbaigi, A., and Pestka, S. (1986) "A Sandwich Radioimmunoassay for Human IFN-a," in Methods in Enzymology, Vol. 119 (S. Pestka, ed.), Academic Press, New York, 582-587.

3. Human IFN-a international reference standard provided by the NIH, reference no. Gxa01-901-535. Pestka, S. (1986) "Interferon Standards and General Abbreviations," in Methods in Enzymology, Vol. 119 (S. Pestka, ed.), Academic Press, New York, 14-23.

This Product Is For Research Use Only. It Is Not For Human Use.


For In Vitro Research Use Only-Not for use in Diagnostics


HUMAN INTERFERON ALPHA (Hu-IFN-a a) MULTI-SUBTYPE ELISA KIT

Product Number: cat#RDI-PB41105

Size: single-plate kit

Lot Number:

Quantity Shipped:

Date Shipped:

Storage Conditions: Keep at 4EC for greatest stability.

Range: 10-500 pg/ml for the high sensitivity protocol. 40-5000 pg/ml for the extended range protocol.

Speed: Incubation time, 3 hours 15 minutes.

Specificity: Human IFN-aA, a2, aA/D, aB2, aC, aD, aG, aH, aI, aJ, aK, a4b, and aWA. No cross-reactivity with human

IFN-g or IFN-b. No cross-reactivity with mouse or rat IFN-a, IFN-b, or IFN-g.

Sample Type: Natural or recombinant human IFN-a in solution or tissue culture media (containing FBS).

Sample Size: 100 ul

Specifications: This kit quantitates human interferon alpha using a sandwich immunoassay.1,2 The kit is based on an ELISA with anti-secondary antibody conjugated to horseradish peroxidase (HRP). Tetramethylbenzidine (TMB) is the substrate. All reagents are supplied. One pre-coated, microtiter breakaway strip plate (96 wells) is included. The specific activity of the interferon used as the standard in the kit is based on the international standard for human interferon alpha (Hu-IFN-�) provided by the National Institutes of Health.3 A typical standard curve for each lot and a microtiter plate worksheet are included with the procedure. Please note that the concentrations of the detecting antibody and the HRP differ from lot to lot. The differences are the result of calibrating each kit for optimal sensitivity. Quality Control: Standard written procedures are followed in the preparation of all ELISA kits. Each group of ELISA kits is assigned a lot number. Sample kits from each lot are retained for periodic evaluation. Kits are guaranteed for six months from the date of production.

1. Staehelin, T., St�hli, C., Hobbs, D.S., and Pestka, S. (1981) "A Rapid Quantitative Assay of High Sensitivity for Human Leukocyte Interferon with Monoclonal Antibodies," in Methods in Enzymology, Vol. 79 (S. Pestka, ed.), Academic Press, New York, 589-595.

2. Kelder, B., Rashidbaigi, A., and Pestka, S. (1986) "A Sandwich Radioimmunoassay for Human IFN-a," in Methods in Enzymology, Vol. 119 (S. Pestka, ed.), Academic Press, New York, 582-587. 3. Human IFN-a international reference standard provided by the NIH, reference no. Gxa01-901-535. Pestka, S. (1986) "Interferon Standards and General Abbreviations," in Methods in Enzymology, Vol. 119 (S. Pestka, ed.),


HUMAN INTERFERON ALPHA (Hu-IFN-a a) FOR HUMAN SERUM SAMPLES ELISA KIT

Product Number: cat#RDI-PB41110    click here for sample pdf protocol

Size: single-plate kit

Price: US$562.00

Lot Number:

Quantity Shipped:

Date Shipped:

Storage Conditions: Keep at 4EC for greatest stability.

Range: 10-500 pg/ml for the high sensitivity protocol. 40-5000 pg/ml for the extended range protocol.

Speed: Incubation time, 3 hours 15 minutes.

Specificity: Human IFN-aA, a2, aA/D, aB2, aC, aD, aG, aH, aI, aJ, aK, a4b, and aWA. No cross-reactivity with

human IFN-g or IFN-b. No cross-reactivity with mouse or rat IFN-a, IFN-b, or IFN-g.

Specifications: This kit quantitates human interferon alpha in human serum samples, as well as other serum and media, using a sandwich immunoassay.1,2 The kit is based on an ELISA with anti-secondary antibody conjugated to horseradish peroxidase (HRP). Tetramethylbenzidine (TMB) is the substrate. All reagents are supplied. One pre-coated, microtiter breakaway strip plate (96 wells) is included. The specific activity of the interferon used as the standard in the kit is based on the international standard for human interferon alpha (Hu-IFN-a) provided by the National Institutes of Health.3 A typical standard curve for each lot and a microtiter plate worksheet are included with the procedure. Please note that the concentrations of the detecting antibody and the HRP differ from lot to lot. The differences are the result of calibrating each kit for optimal sensitivity. Quality Control: Standard written procedures are followed in the preparation of all ELISA kits. Each group of ELISA kits is assigned a lot number. Sample kits from each lot are retained for periodic evaluation. Kits are guaranteed for six months from the date of production.

1. Staehelin, T., St�hli, C., Hobbs, D.S., and Pestka, S. (1981) "A Rapid Quantitative Assay of High Sensitivity for Human Leukocyte Interferon with Monoclonal Antibodies," in Methods in Enzymology, Vol. 79 (S. Pestka, ed.), Academic Press, New York, 589-595.

2. Kelder, B., Rashidbaigi, A., and Pestka, S. (1986) "A Sandwich Radioimmunoassay for Human IFN-a," in Methods in Enzymology, Vol. 119 (S. Pestka, ed.), Academic Press, New York, 582-587.

3. Human IFN-a international reference standard provided by the NIH, reference no. Gxa01-901-535. Pestka, S. (1986) "Interferon Standards and General Abbreviations," in Methods in Enzymology, Vol. 119 (S. Pestka, ed.), Academic Press, New York, 14-23.

This Product Is For Research Use Only. It Is Not For Human Use.


MOUSE INTERFERON ALPHA (Mu-IFN-�) ELISA KIT

Product Number: cat#RDI-PB42100

Size: single-plate kit

Price: US$562.00

Lot Number:

Quantity Shipped:

Date Shipped:

Storage Conditions: Keep at 4�C for greatest stability.

Range: 12.5 � 500 pg/ml

Speed: Overnight incubation (26 hrs. 15 min.)

Specificity: Mouse IFN-�A. The kit has not been tested with other species of mouse interferon alpha.

Specifications: The kit is based on an ELISA with anti-secondary antibody conjugated to horseradish peroxidase (HRP). Tetramethylbenzidine (TMB) is the substrate. All reagents are supplied. One pre-coated, microtiter breakaway strip plate (96 wells) is included. The specific activity of the interferon used as the standard in the kit is based on the international standard for mouse interferon alpha (Mu-IFN-�) provided by the National Institutes of Health.3 A typical standard curve for each lot and a microtiter plate worksheet are included with the procedure. Please note that the concentrations of the detecting antibody and the HRP differ from lot to lot. The differences are the result of calibrating each kit for optimal sensitivity.

Quality Control: Standard written procedures are followed in the preparation of all ELISA kits. Each group of ELISA kits is assigned a lot number. Sample kits from each lot are retained for periodic evaluation. Kits are guaranteed for three months from the date of production (until further stability testing is complete).

1. Staehelin, T., St�hli, C., Hobbs, D.S., and Pestka, S. (1981) "A Rapid Quantitative Assay of High Sensitivity for Human Leukocyte Interferon with Monoclonal Antibodies," in Methods in Enzymology, Vol. 79 (S. Pestka, ed.), Academic Press, New York, 589-595.

2. Kelder, B., Rashidbaigi, A., and Pestka, S. (1986) "A Sandwich Radioimmunoassay for Human IFN-�," in Methods in Enzymology, Vol. 119 (S. Pestka, ed.), Academic Press, New York, 582-587.

3. Human IFN-� international reference standard provided by the NIH, reference no. Gxa01-901-535. Pestka, S. (1986) "Interferon Standards and General Abbreviations," in Methods in Enzymology, Vol. 119 (S. Pestka, ed.), Academic Press, New York, 14-23.


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for recombinant cytokines and antibodies , click here

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EMAIL :margaret@cellular-research.com

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