rev: February 26, 2002


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Human Cytokine/Growth Factor/Chemokines Sample Spec sheets (subject to change without notice):

Recombinant Human sTIE-1/Fc Chimera  (see also sTIE-2/Fc chimera)

Recombinant Human sTIE-1/Fc Chimera

Description: Recombinant human soluble TIE-1 was fused with the Fc part of human IgG1. The recombinant mature sTIE-1/Fc is a disulfide-linked homodimeric protein. The sTIE-1/Fc monomers have a mass of approximately 125 kDa. The soluble receptor protein consists of the full extracellular domain (Leu25-Ser750). TIE-1 (tyrosine kinase with Ig and EGF homology domains 1) and TIE-2/Tek comprise a receptor tyrosine kinase (RTK) subfamily with unique structural characteristics: two immunoglobulin-like domains flanking three epidermal growth factor (EGF)-like domains and followed by three fibronectin type III-like repeats in the extracellular region and a split tyrosine kinase domain in the cytoplasmic region. These receptors are expressed primarily on endothelial and hematopoietic progenitor cells and play critical roles in angiogenesis, vasculogenesis and hematopoiesis. Human TIE-1 cDNA encodes a 1138 amino acid (aa) residue precursor protein with an 24 residue putative signal peptide, a 735 residue extracellular domain and a 354 residue cytoplasmic domain. Two ligands, angiopoietin-1 (Ang1) and angiopoietin-2 (Ang2), which bind TIE-1 with high-affinity have been identified. Ang2 has been reported to act as an antagonist for Ang1. Mice engineered to overexpress Ang2 or to lack Ang1 or Tie-1 display similar angiogenic defects. The recombinant mature TIE-1/ Fc is a disulfide-linked homodimeric protein. Human TIE-1/Fc monomer has a calculated molecular mass of approximately 105 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 125 kDa protein in SDS-PAGE under reducing conditions.

Source:                           Insect cells

Molecular Weight:         250 kDa

Subunit:                          glycosylated dimer

Purity:                            > 90%, by SDS-PAGE and visualised by silver stain

Endotoxin level:             < 0.1 ng per mg sTIE-1/Fc

Stabilizer:                       none

Buffer:                            PBS

Formulation:                   lyophilized

Biological Activity:       Since a ligand for TIE-1 has not yet been identified, the recombinant protein was not tested

                                              for biological activity.

Reconstitution:           The lyophilised sTIE-1/Fc is soluble in water and most aqueous buffers. The lyophilised sTIE-1/

                                    Fc should be reconstituted in PBS or medium to a concentration not lower than 50 µg/ml.

Stability:                  Lyophilised samples are stable for greater than six months at –20°C to –70°C. Reconstituted sTIE-1/

                                Fc should be stored in working aliquots at -20°C. Avoid repeated freeze-thaw cycles!

Usage: sTIE-1/Fc is offered for research use. Not for drug use. Not for human use!

Catalogue number: RDI-SFC011     Size: 100 µg  $440.00    $375.00/vial 5+

Range: 10-100 ng/ml

Literature: [Sato et al., PNAS 90:9355, 1993; Gale et al., Gen Dev 13:1055, 1999]

For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.  It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.

RDI Divison of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049


phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266


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