rev:  November 18, 2004


Return (human cytokine index)

Human Cytokine/Growth Factor/Chemokines Sample Spec sheets (subject to change without notice):

Recombinant Human sBMPR-1A
Recombinant Human soluble BMPR-IA

Description: The extracellular domain of human BMPR-IA was fused with a carboxy -terminal 6X histidine-tag. The monomeric glycoprotein was expressed in baculovirus infected insect cells. Cellular responses to bone morphogenetic proteins (BMPs) have been shown to be mediated by the formation of hetero-oligomeric complexes of the type I and type II serine/threonine kinase receptors. BMP receptor 1A (BMPR-1A), also known as activin receptor-like kinase (ALK)-3, is a one of seven known type I serine/threonine kinases that are required for the signal transduction of TGF-b family cytokines. In contrast to the TGF -b receptor system in which the type I receptor does not bind TGF-b in the absence of the type II receptor, type I receptors involved in BMP signaling (including BMPR-IA, BMPR-IB/ALK-6, and ActR-I/ALK-2) can independently bind the various BMP family proteins in the absence of type II receptors. Recombinant soluble BMPR-IA binds BMP-2 and -4 with high-affinity in solution and is a potent BMP-2/4 antagonist in vitro. BMPR-IA is ubiquitously expressed during embryogenesis. In adult tissues, BMPR-IA mRNA is also widely distributed; with the highest expression levels found in skeletal muscle. The extracellular domain of BMPR-IA shares little amino acid sequence identity with the other mammalian ALK type I receptor kinases, but the cysteine residues are conserved. Human and mouse BMPR-IA are highly conserved and share 98% sequence identity.

Source:                                   Insect cells

Molecular Weight:                 23 kDa

Subunit:                                  glycosylated monomer

Purity:                                    >90%, by SDS-PAGE and visualised by silver stain

Endotoxin level:                     < 0.1 ng per mg of sBMPR-IA

Stabilizer:                               HSA

Buffer:                                     PBS

Formulation:                            lyophilized

Biological Activity: Measured by its ability to inhibit recombinant human BMP-2 I induced alkaline phosphatase production by C2C12 myogenic cells. The ED50 for this effect is typically 1-3 µg/ml in the presence of 500 ng/ml of BMP-2.

Reconstitution: The lyophilised sBMPR-IA should be reconstituted in PBS or medium containing at least 0.1% human or bovine serum albumin to a concentration not lower than 50 µg/ml.

Stability: Lyophilised samples are stable for greater than six month at -20°C to -70°C. Upon reconstitution, this cytokine can be stored under sterile conditions at -2°C to -4°C for one month or at -20°C to -70°C for three month without detectable loss of activity. Avoid repeated freeze-thaw cycles!

Usage: sBMPR-IA is offered for research use. Not for drug use. Not for human use!

Catalogue number: RDI-S01-021  Size: 100 µg  $625.00       $562.00/5+

Range: 1-4 µg/ml

Literature: [Kirsch et al., FEBS 468 :215, 2000 ; Ten Dijke et al., Oncogene 8:2879, 1993; Kawabal et al.,

Cytokine Growth Factor Rev 9:49, 1998; Ebendal et al., J Neuroscience Res 51:139, 1998]

For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.  It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.

RDI Divison of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049


phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

Ordering terms


Return (human cytokine index)

all rights reserved by any/all copyright holders?