rev:     November 11, 2004

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HUMAN Cytokine/Growth Factor/Chemokines Sample Spec sheets (subject to change without notice):

DATA SHEET: gAcrp30 is a naturally occurring globular protein, obtained by proteolytic processing of adiponectin. Adiponectin is produced and secreted exclusively by adipocytes, and is a relatively abundant plasma protein, accounting for up to 0.05% of total serum protein. Like Adiponectin, gAcrp30 is capable of decreasing hyperglycemia and reversing insulin resistance. Additionally, gAcrp30 has been shown to be an important factor in promoting fat loss by signaling muscle to absorb and burn Free-Fatty Acids (FFAs). The signaling receptors for adiponectin and gAcrp30 have recently been identified and names AdipoR1 and AdipoR2. AdipoR2 is predominantly expressed in the liver. This recombinant human gAcrp30/Adipolean is a 16.6 kDa protein consisting of 145 amino acid residues

Catalog#: RDI-4521

Package Size: 25 micrograms $231.00   $219.00/pack 2+

               Mini-Pack:cat#RDI-4521P $94.00/5ug

Supplied: The sterile filtered solution was lyophilized from 10mM Tris, pH 9.2 + 0.05% Sarcosyl + 0.75mM DTT

Source: E. Coli

Purity: >98% by SDS-Page analysis Endotoxin <1EU/ug

Reconstitution: The lyophilized human gACRp30 should be reconstituted in water to a concentration of <0.3mg/ml. Allow to set at least 30 minutes at 4-8 DEG C, Mix well. If desired, centrifuge for 1-2 minutes at 1000 rpm to concentrate material in vial.This solution can be diluted into other buffered solutions and stored at 4 DEG C for up to 1 week or stored at -20 DEG C for future use.

Note: If this material is not to be used for coating or labeling (or other applications requiring carrier free material), the addition of 0.1% BSA to dilution buffers or dilution in sterile culture media is recommended to enhance stability and minimize absorption to vial. Ideally, maintain stock >10ug/ml.

Storage: The lyophilized powder is stable at room temperature for a few weeks but it is best stored desiccated at -20 Deg C. Reconstituted material should be stored in working aliquots at -20DEG C. AVOID FREQUENT FREEZE THAW CYCLES.

Reactivity: determined by its abaility to inhibit the proliferation of murine myeloid cell lines M1. the ED50 for this effect is <3ug/ml, corresponding to a specific activity of 3.3 X 10 E05 units/mg

AA Sequence:

MKGEPGEGAY VYRSAFSVGL ETYVTIPNMP IRFTKIFYNQ QNHYDGSTGK

FHCNIPGLYY FAYHITVYMK DVKVSLFKKD KAMLFTYDQY QENNVDQASG

SVLLHLEVGD QVWLQVYGEG ERNGLYADND NDSTFTGFLL YHDTN

Goat anti-human Adipolean (antigen aff pure, lyophilized, no carrier/no preservative)

cat#RDI-ADIPOLabGP       $250.00/50ug

cat#RDI-ADIPOLabGP1    $375.00/100ug

Goat anti-human Adipolean:Biotin conjugated (antigen aff pure, conjugated to Biotin, lyophilized, carrier free)

cat#RDI-ADIPOLabGPB     $250.00/25ug

cat#RDI-ADIPOLabGPB1   $375.00/50ug


Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.  It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent  infringements that might result with the use of or derivation of this product.

-see also recombinant murineAdipolean  and recombinant human adipolean variant

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