rev: May 13, 2005
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Human Cytokine/Growth Factor/Chemokines Sample Spec sheets (subject to change without notice):
mini-pack cat#RDI-4517P $94.00/2ug
Supplied: The sterile filtered solution was lyophilized from 10 mM Sodium Citrate pH 4.5 + 150 mM NaCl.
Source: E. Coli
Purity: >98% by SDS-Page analysis Endotoxin <1EU/ug
Reconstitution: The lyophilized Artemin should be reconstituted in water to a concentration of 0.1-0.5mg/ml. Allow to set at least 30 minutes at 4-8 DEG C, Mix well. If desired, centrifuge for 1-2 minutes at 1000 rpm to concentrate material in vial.This solution can be diluted into other buffered solutions and stored at 4 DEG C for up to 1 week or stored at -20 DEG C for future use.
Note: If this material is not to be used for coating or labeling (or other applications requiring carrier free material), the addition of 0.1% BSA to dilution buffers or dilution in sterile culture media is recommended to enhance stability and minimize absorption to vial. Ideally, maintain stock >50ug/ml.
Storage: The lyophilized powder is stable at room temperature for a few weeks but it is best stored desiccated at -20 Deg C. Reconstituted Artemin should be stored in working aliquots at -20DEG C. AVOID FREQUENT FREEZE THAW CYCLES.
Reactivity: The ED50 as determined by its ability to stimulate the proliferation of human SH-SY5Y cells is 2-5ng/ml.
-Assay#2: (in testing): determined by its ability to promote neuronal survival and neurite outgrowth on dorsal root ganglion neurons
AGGPGSRARA AGARGCRLRS QLVPVRALGL GHRSDELVRF RFCSGSCRRA
RSPHDLSLAS LLGAGALRPP PGSRPVSQPC CRPTRYEAVS FMDVNSTWRT VDRLSATACG CLG
Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.
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RDI Div of Fitzgerald Industries Intl
(Research Diagnostics Inc)
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Concord MA 01742-3049
phone (978) 371-6446 or (800) 370-2222
fax (978) 371-2266
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