rev: May 13, 2005
Return (RDI HOME PAGE)
Return (human cytokine index)
Human Cytokine/Growth Factor/Chemokines Sample Spec sheets (subject to change without notice):
Persephin is a disulfide-linked homodimer neurotrophic factor structurally
related to GDNF, Artemin, and Neurturin. These proteins belong to the
cysteine-knot family of growth factors that assume stable dimeric structures.
Persephin signals through a multicomponent receptor system, composed of RET
and one of four GFR alpha (alpha1-alpha4) receptors. The GFRalpha4 was first
identified in chicken and was later shown to be the preferential binding
subunit for Persephin. Persephin promotes the survival of ventral midbrain
dompaminergic neurons and motor neurons after sciatic nerve oxotomy, and
like GNDF, promotes ureteric bud branching. However, in contrast to GDNF
and Neurturin, Persephin does not support survival of peripheral neurons.
Recombinant human Persephin is a disulfide-linked homodimer, composed of
two 10.3 kDa polypeptide chains (192 total amino acid residues). Each chain
contains seven conserved cysteine residues, one of which (Cys 63) is used
for inter-chain disulfide bridging and the others are involved in intramolecular
ring formation known as the cysteine knot configuration.
Package Size: 20 micrograms $231.00 $219.00/pack 2+
Bulk quotes on request
Supplied: Lyophilized protein from 3mM PBS pH7.2, with no additives.
Source: E. Coli
Purity: >98% by SDS-Page analysis. Endotoxin level is less than 0.1ng per ug (1EU/ug) of human PSP.
Reconstitution: The lyophilized PSP is soluble in water and most aqueous buffers. The lyophilized powder should be reconstituted sterile DI water to a concentration of 0.1-1mg/ml. Allow to set at least 30 minutes at 4 DEG C. If desired, can centrifuge for 1 minute at 1000 rpm to concentrate material in vial. This solution can be diluted into water or other buffered solutions or stored at 4 DEG C for up to 1 week or at -20 DEG C for future use.
Note:If this material is not to be used for coating or labeling (or other applications requiring carrier free material), the addition of 0.1% BSA to dilution buffers or dilution in sterile culture media is recommended to enhance stability and minimize absorption to vial. Ideally, maintain stock >50ug/ml.
Storage: The lyophilized powder, though stable at room temperature for a few weeks, is best stored desiccated at -20 DEG C. Reconstituted PSP should be stored in working aliquots at -20 DEG C. Avoid Frequent Freeze-THAW Cycles.
Reactivity: Human Persephin induces RET phosphorylation using a concentration range of 0.1-1.0ng/ml. Human persephin binds to mammalian GFRa4 with the Kd of 100pM. Other members of the GDBF family (Artemin, GDNF and Neurturin) do not bind to mammalian GFRa4.
ALSGPCQLWS LTLSVAELGL GYASEEKVIF RYCAGSCPRG
ARTQHGLALA RLQGQGRAHG GPCCRPTRYT DVAFLDDRHR
Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.
Return (RDI HOME PAGE)
RDI Div of Fitzgerald Industries Intl
(Research Diagnostics Inc)
34 Junction Square Drive
Concord MA 01742-3049
phone (978) 371-6446 or (800) 370-2222
fax (978) 371-2266
all rights reserved by any/all copyright holders?