rev: October 20, 2004
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Murine/Rat Cytokine/Growth Factor/Chemokines Sample Spec sheets (subject to change without notice):
-Fibroblast Growth Factor-9 (FGF-9, Glial Activating Factor) is a heparin binding factor which stimulates the proliferation and activation of glial cells and other cells which express FGF receptors. Murine FGF-9 is a 23.3kDa protein containing 205 amino acid residues.
Catalog#: RDI-4505 or RDI-4503
Batch# see individual batch sheet with shipment
Package Size: 10 micrograms $231.00 $219.00/vial 2+
Mini-Pack: cat#RDI-4505P $94.00/2ug
Supplied: Lyophilized powder from 20mM Tris poH8.0 + 75mM NaCl.
Source: E. Coli
Purity: >98% by SDS-Page analysis and N-terminal sequence analysis. Endotoxin
level is less than 0.1ng per ug of murine FGF-9.
Reconstitution:The lyophilized FGF-9 should be reconstituted in sterile 40mM Tris pH8.0 buffer to a concentration of 1mg/ml . Allow to set at least 30 minutes at 4 DEG C , tighten cap and rotate to reconstitute any material on sides of vial. If desired, you can centrifuge at 1000 rpm for 1 minute to concentrate volume in bottom of vial. This solution can be diluted into other buffered solutions or stored at 4 DEG C for up to 1 week or at -20 Deg C for future use.Repeated Freeze thaw cycles will result in loss of activity.
Storage: The lyophilized powder is stable for a few weeks at room temperature but it is best stored desiccated at -20 DEG C. Reconstituted FGF-9 should be stored in working aliquots at -20 DEG C. AVOID FREQUENT FREEZE THAW CYCLES.
Note:If this material is not to be used for coating or labeling (or other applications requiring carrier free material), the addition of 0.1% BSA to dilution buffers or dilution in sterile culture media is recommended to enhance stability and minimize absorption to vial. Ideally, maintain stock >20ug/ml.
Reactivity: The ED50 as determined by the dose dependent stimulation of thymidine uptake by Baf3 cells expressing FGF receptors is less than 0.5ng/ml, corresponding to a specific activity of >2 million units per mg.
AA Sequence :
PLGEVGSYFG VQDAVPFGNV PVLPVDSPVL LNDHLGQSEA
GGLPRGPAVT DLDHLKGILR RRQLYCRTGF HLEIFPNGTI
QGTRKDHSRF GILEFISIAV GLVSIRGVDS GLYLGMNEKG
ELYGSEKLTQ ECVFREQFEE NWYNTYSSNL YKHVDTGRRY
YVALNKDGTP REGTRTKRHQ KFTHFLPRPV DPDKVPELYK DILSQS
Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.
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