rev: 5/18/2008
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Murine/Rat Cytokine/Growth Factor/Chemokines
Sample Spec sheets (subject to change without notice):
DATA SHEET:recombinant RAT INTERLEUKIN-15
-IL-15 is an immunomodulating cytokine that shares many biological
properties of IL-2. They share common receptor subunits, the beta and gamma
chains of the IL-2R, resulting in crucial functions in the development,
survival and activation of NK and T cells. IL-15 is a co-stimulator for
proliferation of PMA or anti-IgM activated purified B cells. Unlike IL-2, IL-15
acts as an agonist of human neutrophils and proliferates mouse mast cells and
bone marrow-derived mouse mast cells. Increased expression of IL-15 has been
reported in patients with rheumatoid arthritis, inflammatory bowel disease and
diseases affiliated with retroviruses HIV and HTLV-I. This recombinant Rat IL-15
is a 13.5 kDa protein, consisting of 115 amino acid residues.
Catalog#: RDI-4024
Package Size:
10 micrograms $258.50
-Mini-Pack# cat#RDI-4024p $94.00/2ug
Supplied: Lyophilized powder with no additives.
Source: E. Coli
Purity: >96% by SDS-Page and HPLC analysis. Endotoxin level is less than
0.1ng per ug (1EU/ug) of rat IL-15
Reconstitution: At first use, tighten cap, genetly tap on counter or centrifuge 1 minute 1000 rpm to concnetrate powder in bottom of vial. The lyophilized IL-15 should then be reconstituted in 50mM Tris pH8.5 to a concentration of 0.1- 1mg/ml. Allow to set at least 30 minutes at 4 DEG C, Mix well. If desired, centrifuge for 1-2 minutes at 1000 rpm to concentrate material in vial. This solution can be diluted into other buffered solutions or stored at 4 DEG C for 1 week or -20 DEG C for future use.
Note:If this material is not to be used for coating or labeling (or other applications requiring carrier free material), the addition of 0.1% BSA to later dilution buffers or dilution in sterile culture media is recommended to enhance stability and minimize absorption to vial. Ideally, maintain stock >10ug/ml.
Storage: The lyophilized powder is stable at room temperature for a few weeks but it is best stored desiccated at - 20 DEG C. Reconstituted IL-15 should be stored in working aliquots at -20 DEG C. AVOID FREQUENT FREEZE THAW CYCLES.
Reactivity: The ED50 as determined by the stimulation of the proliferation of murine CTLL-2 cells is <10ng/ml corresponding to a specific activity of > 100K units/mg.
AA Sequence:
MNWIDVRYDL ELIESLIQFI HIDTTLYTDS
DFHPSCKVTA MNCFLLELQV ILHEYSNMTL
NETVRNLYL ANSTLSSNKN VIESGCKECE
ELEERNFTEF LQSFIHIVQM FINTS
Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.
see also mouse IL-15 and human IL15 and antibodies to IL15
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