rev: October 20, 2004
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Murine/Rat Cytokine/Growth Factor/Chemokines Sample Spec sheets (subject to change without notice):
see also mouse GM-CSF and human GM-CSF
DATA SHEET:recombinant RAT GRANULOCYTE MACROPHAGE COLONY
STIMULATING FACTOR (GM-CSF)
Granulocyte Macrophage Colony Stimulating Factor (GM-CSF) is a potent species specific stimulator of bone marrow cells. GM-CSF stimulates precursor cells of granulocytes, macrophages and eosinophils. This recombinant GM-CSF is a 14.5 kDa protein containing 128 amino acid residues.
Catalog#: RDI-4023 lot#
Package: $231.00/package $219.00/package 2+
Mini-Pack:cat#RDI-4023p $94.00/2ug Bulk quotes on request
Package Size: micrograms
Supplied: Lyophilized protein from 10mM TRIS pH8.5 with no additives.
Source: E. Coli
Purity: >98% by SDS-Page analysis. Endotoxin level is less than 0.1ng/ug
(1EU/ug) of rat GM-CSF.
Reconstitution: First tighten cap and gently tap on table or centrifuge 10 seconds 1000RPM to settle contents in vial. The lyophilized GM-CSF is soluble in water and most aqueous buffers. The lyophilized protein should be reconstituted in water to a concentration of 0.1-1mg/ml. Allow to set at least 30 minutes at 4 DEG C , tighten cap and rotate to reconstitute any material on sides of vial. If desired, you can centrifuge at 1000 rpm for 1 minute to concentrate volume in bottom of vial. This solution can be diluted into water or other buffered solutions or stored at 4 DEG C for up to 1 week or at -20 DEG C for future use.
Note:If this material is not to be used for coating or labeling (or other applications requiring carrier free material), the addition of 0.1% BSA or dilution in sterile culture media is recommended to enhance stability and minimize absorption to vial.
Storage: The lyophilized powder, though stable at room temperature for a few weeks, is best stored dessicated dessicated at -20 DEG C. Reconstituted GM-CSF should be stored in working aliquots at -20 DEG C. Avoid Frequent Freeze-THAW Cycles.
Reactivity: The Ed50 as determined by the dose dependent stimulation of the proliferation of murine FDC-P1 cells is <0.01ng/ml,which yields a specific activity of >100 million units/mg (1 X 10 E08)
AA Sequence:
MAPTRSPITV TRPWKHVDAI KEALSLLNDM
RALENEKNGD VDIISNGFSI QRPTCVQTRL
KLYKQGLRGN LTKLNGALTM IASHYQTNCP
PTPETDCEIE VTTFEDFIKN LKGFLFDIPF
DCWKPVQK
Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.
see also mouse GM-CSF (RDI-3153) , human GM-CSF (RDI-303 - $235.00/10µg) , rat GM-CSF(RDI-4023)
and murine M-CSF (RDI-3152)
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