rev:     October 20, 2004

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Murine/Rat Cytokine/Growth Factor/Chemokines Sample Spec sheets (subject to change without notice):


DATA SHEET:recombinant RAT STEM CELL FACTOR

-Stem Cell Factor(SCF) is a hematopoietic growth factor that exerts its activity at the early stages of hematopoiesis. SCF stimulates the proliferation of myeloid, erythroid, and lymphoid progenitors in bone  marrow cultures and has been shown to act synergistically with colony stimulating factors. RAT SCF is an 18.4 kDa protein containing 154 amino acid residues.

Catalog#: RDI-4022      

Package Size: 10 micrograms  $231.00   $219.00/package 2 or more

                    mini-pack RDI-4022P $94.00/2ug       Bulk quotes on request

Supplied: Lyophilized powder from 20mM Tris pH7.5 solution . No carrier/additives.

Source: E. Coli

Purity: >98% by SDS-Page analysis and HPLC analysis. Endotoxin level is less than 0.1ng per ug (1EU/ug) of   SCF.

Reconstitution: The lyophilized SCF should be reconstituted in sterile DI water to a maximum concentration of 0.1- 1mg/ml. Allow to set at least 30 minutes at 4 DEG C , tighten cap and rotate to reconstitute any material on sides of vial. If desired, you can centrifuge at 1000 rpm for 1 minute to concentrate volume in bottom of vial. This solution can be diluted into other buffered solutions containing a carrier protein (as 0.1% BSA) or media containing serum or stored at 4 DEG C for up to 1 week or at -20 DEG C for future use.

Note:If this material is not to be used for coating or labeling (or other applications requiring carrier free material), the addition of 0.1% BSA or dilution in sterile culture media is recommended to enhance stability and minimize absorption to vial.

Storage: The lyophilized powder is stable at room temperature for a few weeks but it is best stored desiccated at -20 DEG C. Reconstituted SCF should be stored in working aliquots at -20 DEG C. AVOID FREQUENT FREEZE THAW CYCLES.

Reactivity: The ED50 as determined by the dose-dependent proliferation of the human M07e cell line is <10ng/ml, corresponding to a specific activity of >1 X 10 E05 units/mg.

AA Sequence:

MQEICRNPVT DNVKDITKLV ANLPNDYMIT LNYVAGMDVL PSHCWLRDMV

THLSVSLTTL LDKFSNISEG LSNYSIIDKL GKIVDDLVAC MEENAPKNVK

ESLKKPETRN FTPEEFFSIF NRSIDAFKDF MVASDTSDCV LSSTLGPEKD

SRVSVTKPFM LPPVA

Precautions: For In vitro research Use Only. Not for use   in or on humans or animals or for diagnostics.  It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.


see also human SCF and murine SCF

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