rev:   October 20, 2004

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MOUSE/RAT Cytokine/Growth Factor/Chemokines Sample Spec sheets (subject to change without notice):


DATA SHEET:recombinant RAT LEPTIN

-Leptin (Anti-Obesity Protein) is the protein product of the ob (obese) ene in mice and seems to be involved in appetite control. The ob gene is expressed in adipose tissue and is thought to regulate the body's fat stores. Mice with the ob/ob genotype also develop a form of diabetes similar to type II (non insulin dependent) diabetes. This recombinant rat Leptin is a 16.2 kDa protein containing 147 amino acid residues.

Catalog#: RDI-4021 lot#1003211

Package Size: 1 milligram $231.00  $219.00/pack 2 or more  

Supplied: Lyophilized powder from solution 0.1% TFA with no additives.

Source: E. Coli

Purity: >95% by SDS-Page analysis and HPLC analysis. Endotoxin level is less than 0.1ng per ug (1Eu/ug) of rat leptin.

Reconstitution: The lyophilized leptin is poorly soluble at** neutral pH but can be dissolved in water to a concentration of 1-5mg/ml. This solution can be stored at 4 DEG C for up to 1 week or at -20 DEG C long term.

Storage: The lyophilized powder is stable at room temperature for a few weeks but it is best stored desiccated at -20 DEG C . Reconstituted Leptin should be stored in working aliquots at -20 DEG C. AVOID FREQUENT FREEZE THAW CYCLES.

Reactivity: in testing

AA sequence:

MVPIHKVQDD TKTLIKTIVT RINDISHTQS VSARQRVTGL DFIPGLHPIL

SLSKMDQTLA VYQQILTSLP SQNVLQIAHD LENLRDLLHL LAFSKSCSLP

QTRGLQKPES LDGVLEASLY STEVVALSRL QGSLQDILQQ LDLSPEC

Precautions: For In vitro research Use Only.

A280: about 0.157.

Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.  It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.


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