rev: May 25, 2005
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MOUSE/RAT Cytokine/Growth Factor/Chemokines Sample Spec sheets (subject to change without notice):
IFN-gamma is an acid-labile interferon produced by CD4 and CD8 T lymphocytes as well as activated NK cells. IFN-gamma receptors are present in most immune cells, which respond to IFN-gamma signaling by increasing the surface expression of class I MHC proteins. This promotes the presentation of antigen to T-helper (CD4+) cells. IFN-gamma signaling in antigen-presenting cells and antigen-recognizing B and T lymphocytes regulate the antigen-specific phases of the immune response. Additionally, IFN-gamma stimulates a number of lymphoid cell functions including the anti-microbial and anti-tumor responses of macrophages, NK cells, and neutrophils. Human IFN-gamma is species-specific and is biologically active only in human and primate cells. Recombinant rat IFN-gamma is a 15.6 kDa protein containing 135 amino acid residues.
Catalog#: RDI-4020
Package Size: 100 micrograms $231.00/vial $219.00/package 2+
Mini-Pack:cat#RDI-4020p $94.00/20ug Bulk quotes on request
Supplied: sterile filtered from 1X PBS ,then Lyophilized protein with no
additives.
Source: E. Coli
Purity: >97% by SDS-Page analysis and HPLC analysis. Endotoxin level is
less than 0.1ng per ug (1EU/ug) of IFN-gamma.
Reconstitution: The lyophilized rat IFN-g is soluble in water and most aqueous buffers. The lyophilized powder should be reconstituted in 5mM Tris pH 8.0 & 100mM NaCl to a concentration of 1ug/ul. Let sit at least 30 minutes, tighten cap, rotate vial to reconstitute any material on sides of vial. This material can be diluted into water or other buffered solutions or stored at 4 DEG C for up to 1 week or at -20 DEG C for future use. It is recommended that further dilutions of the stock be made into buffercontaining carrier protein or media containing serum.
Note:If this material is not to be used for coating or labeling (or other applications requiring carrier free material), the addition of 0.1% BSA to dilution buffers or dilution in sterile culture media is recommended to enhance stability and minimize absorption to vial. Ideally, maintain stock >10ug/ml.
Storage: The lyophilized powder, though stable at room temperature for a few weeks, is best stored desiccated at -20 DEG C. Reconstituted material should be stored in working aliquots at -20 DEG C. Avoid Frequent Freeze-THAW Cycles.
Reactivity: The ED50 as determined by a cytopathic affect inhibition assay with murine L929 cells challenged with EMC virus was <0.1ng/ml, which corresponds to a specific activity of >1 X 10 E07 units/mg .
AA Sequence:
MQGTLIESLE SLKNYFNSSS MDAMEGKSLL LDIWRNWQKD
GNTKILESQI ISFYLRLFEV LKDNQAISNN ISVIESHLIT
NFFSNSKAKK DAFMSIAKFE VNNPQIQHKA VNELIRVIHQ
LSPESSLRKR KRSRC
Precautions: For In vitro research Use Only. Not for use in or on humans
or animals or for diagnostics. It is the responsibility of the user
to comply with all local/state and Federal rules in the use of this product.
We are not responsible for any patent infringements that might result with
the use of or derivation of this product.
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RDI Div of Fitzgerlad Industries Intl
(Research Diagnostics Inc)
34 Junction Square Drive
Concord MA 01742-3049
USA
phone (978) 371-6446 or (800) 370-2222
fax (978) 371-2266
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