rev: October 20, 2004
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Murine/Rat Cytokine/Growth Factor/Chemokines Sample Spec sheets (subject to change without notice):
-Rat GRO-B (Rat MIP2) promotes neutrophil chemotaxis and degranulation.
Recombinant Rat GRO-beta/MIP-2 is a 7.9 kDa protein containing 73 amino acid
residues
Catalog#: RDI-4011
Package Size: 25 micrograms $231.00 $219.00/package 2+
Mini-Pack: cat#RDI-4011P $94.00/5ug Bulk quotes on request
Supplied: Lyophilized powder with no additives.
Source: E. Coli
Purity: >98% by SDS-Page analysis. Endotoxin <1EU/ug.
Reconstitution: The lyophilized rat GRO-B/MIP-2 should be reconstituted in
water to a concentration of 0.1- 1.0mg/ml. Allow to set at least 30 minutes
at 4 DEG C, tighten cap and rotate to reconstitute any material on sides
of vial. If desired, you can centrifuge at 1000 rpm for 1 minute to concentrate
volume in bottom of vial. This solution can be diluted into other buffered
solutions containing carrier protein or medium and stored at 4 DEG C for
up to 1 week or long term at -20 DEG C for future use.
Note:If this material is not to be used for coating or labeling (or other applications requiring carrier free material), the addition of 0.1% BSA to dilution buffers or dilution in sterile culture media is recommended to enhance stability and minimize absorption to vial. Ideally, maintain stock >50ug/ml.
Storage: The lyophilized powder is stable at room temperature for a few weeks but it is best stored desiccated at -20 Deg C. Reconstituted GRO-B should be stored in working aliquots at -20 DEG C. AVOID FREQUENT FREEZE THAW CYCLES.
Reactivity: Determined by its ability to chemoattract rat neutrophils using a concentration range of 10.0-100.0 ng/ml.
AA Sequence:
VVVASELRCQ CLTTLPRVDF KNIQSLTVTP PGPHCAQTEV
IATLKDGHEV CLNPEAPLVQ RIVQKILNKG KAN
Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.
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