rev: October 20, 2004
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Murine/Rat Cytokine/Growth Factor/Chemokines Sample Spec sheets (subject to change without notice):
-Rat GRO (Rat KC, CINC) promotes neutrophil chemotaxis and degranulation.
Rat GRO is an 8 kDa protein containing 72 amino acid residues.
Catalog#: RDI-4010
Batch# see individual batch sheet with shipment
Package Size: 25 micrograms $231.00 $219.00/pack 2+
Mini-PacK:cat#RDi-4010P $94.00/5ug
Bulk quotes on request
Supplied: Lyophilized powder with no additives.
Source: E. Coli
Purity: >98% by SDS-Page analysis.
Reconstitution: The lyophilized Rat GRO should be reconstituted in sterile
water to a concentration of 100ng/ul. Allow to set at least 30 minutes at
4 DEG C, tighten cap and rotate to reconstitute any material on sides of
vial. If desired, you can centrifuge at 1000 rpm for 1 minute to concentrate
volume in bottom of vial. This solution can be diluted into other buffered
solutions .
Note:If this material is not to be used for coating or labeling (or other
applications requiring carrier free material), the addition of 0.1% BSA to
dilution buffers or dilution in sterile culture media is recommended to enhance
stability and minimize absorption to vial. Ideally, maintain stock
>50ug/ml.
Storage: The lyophilized powder is stable at room temperature but it is best
stored desiccated below 0 Deg C. Reconstituted GRO should be stored in working
aliquots at -20 DEG C. AVOID FREQUENT FREEZE THAW CYCLES.
Reactivity: The rat GRO showed maximum chemotactic activity on rat neutrophils
as assayed in a modified Boyden chamber at 100ng/ml.
Precautions: For In vitro research Use Only. Not for use in or on humans
or animals or for diagnostics. It is the responsibility of the user
to comply with all local/state and Federal rules in the use of this product.
We are not responsible for any patent infringements that might result with
the use of or derivation of this product.
Catalog#: RDI-RTGROKCabr
Package Size: 500 micrograms $250.00/vial
Supplied: Lyophilized powder with no additives.
Source: rabbit
Purity: IgG purified via Protein A chromatography
Reconstitution: Reconstitute 500ul of sterile distilled water to yield a
1mg/ml solution in PBS. This solution can be further diluted into other buffered
solutions or stored at -20 DEG C for future use. The antibody must be titered
for optimal use. For most applications the dilution will be between
1:500 - 1:5000.
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Activity: A 1:1000 dilution will give satisfactory reactivity in a direct
Elisa when 1ug/ml Rat GRO/KC is used for coating the plate.
Application -Neutralization
-RIA/ ELISA (approx 1ug/ml as coating ab)
-All
titers must be determined in assay
Precautions: For In vitro research Use Only. Not for usein or on humans or
animals or for diagnostics. It is the responsibility of the
user to comply with all local/state and Federal rules in the use of this
product. We are not responsible for any patent infringements that might result
with the use of or derivation of this product.
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