rev:   October 20, 2004

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Murine/Rat Cytokine/Growth Factor/Chemokines Sample Spec sheets (subject to change without notice):


DATA SHEET:recombinant RAT INTERLEUKIN-7

-IL-7 is a hematopoietic growth factor produced predominantly by epithelial cells, especially keratinocytes and thymic epithelial cells. It is a single chain glycoprotein of 25.0 kDa, which contains the three disulfide bonds that are essential for biological activity. IL-7 is a potent lymphoid cell growth factor which affects Pre-B, Pro-B and early T cells. It will also stimulate proliferation and differentiation of mature T cells in combination with other factors such as IL-2. Recombinant Rat IL-7 is a 15.0 kDa protein consisting of 130 amino acid residues.

Catalog#: RDI-4007

Package Size: 10 micrograms $231.00   $219.00/package 2+

                    MINI-PACK:cat#RDI-4007P $94.00/2ug      Bulk quotes on request

Supplied: Lyophilized powder from 10mM sodium acetate pH5.8 with no additives

Source: E. Coli

Purity: >98% by SDS-Page analysis and HPLC analysis. Endotoxin level less than 0.1ng/ug (1EU/ug)

Reconstitution: We recommend a quick spin followed by reconstitution in sterile DI water to a concentration of 0.1-1.0 mg/ml. Allow to set at least 30 minutes at 4 DEG C, tighten cap and rotate to reconstitute any material on sides of vial. If desired, you can centrifuge at 1000 rpm for 1 minute to concentrate volume in bottom of vial. This solution can be diluted into other buffered solutions containing carrier protein or medium containing serum and stored at 4 DEG C (up to 1 week) or -20 DEG C long term)

Note: If this material is not to be used for coating or labeling or other applications requiring carrier free material), the addition of 0.1% BSA to dilution buffers or dilution in sterile culture media is recommended to enhance stability and minimize absorption to vial. Ideally, maintain stock >10ug/ml.

Storage: The lyophilized powder is stable at room temperature for a few weeks but it is best stored desiccated at -20 Deg C. Reconstituted IL-7 should be stored in working aliquots at -20 DEG C . AVOID FREQUENT FREEZE THAW CYCLES.

Biological  Activity : Determined by the dose-dependant stimulation of   the proliferation of murine 2E8 cells. The   expected ED50 for this effect is 1.0-2.0 ng/ml.

AA Sequence :

MDCHIKDKDG KAFGSVLMIS INQLDKMTGT DSDCPNNEPN

FFKKHLCDDT KEAAFLNRAA RKLRQFLKMN ISEEFNDHLL

RVSDGTQTLV NCTSKEEKTI KEQKKNDPCF LKRLLREIKT

CWNKILKGSI

Precautions: For In vitro research Use Only. Not for use in or on humans or animals   or for diagnostics.  It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.


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