rev:     October 20, 2004

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Murine/Rat Cytokine/Growth Factor/Chemokines Sample Spec sheets (subject to change without notice):


DATA SHEET:recombinant RAT INTERLEUKIN-13

-IL-13 is an immunoregulatory cytokine produced mainly by T helper 2 cells, but also by mast cells and NK cells. IL-13 is a unique cytokine having structural features and functional characteristics with IL-4. However, it only shares about 30% homology with the IL-4 sequence. IL-13 inhibits IL-1 beta, IL-6, IL-8, and TNF-alpha resulting in anti-inflammatory effects on monocytes and macrophages. It also induces B cells to proliferate and isotype-switch to the production of IgE. In addition, IL-13 modulates TH2 cell development interfering key roles in the ostracizing of gastrointestinal parasites and in the evolvement of asthma and allergy. This recombinant Rat IL-13 is an 11.9 kDa protein containing 109 amino acid residues.

Catalog#: RDI-4006

Package Size: 10 micrograms $231.00   $219.00/package 2+

                       MINI-PACK:cat#RDI-4006P  $94.00/2ug      Bulk quotes on request

Supplied: Lyophilized powder with no additives

Source: E. Coli

Purity: >96% by SDS-Page analysis and HPLC analysis. Endotoxin level less than 0.1ng/ug (1EU/ug)

Reconstitution: We recommend a quick spin followed by reconstitution in sterile DI water to a concentration of 0.1-1.0 mg/ml. Allow to set at least 30 minutes at 4 DEG C, tighten cap and rotate to reconstitute any material on sides of vial. If desired, you can centrifuge at 1000 rpm for 1 minute to concentrate volume in bottom of vial. This solution can be diluted into other buffered solutions containing carrier protein or medium containing serum and stored at 4 DEG C (up to 1 week) or -20 DEG C long term)

Storage: The lyophilized powder is stable at room temperature for a few weeks but it is best stored desiccated at -20 Deg C. Reconstituted IL-13 should be stored in working aliquots at -20 DEG C . AVOID FREQUENT FREEZE THAW CYCLES.

Reactivity: The ED50 as determined by the dose-dependant stimulation of the proliferation of human TF-1 cells is < 40 ug/ml, corresponding to a specific activity of > 2.5 x 10 E04 units/mg.

AA Sequence:

VRRSTSPPVA LRELIEELSN ITQDQKTSLC NSSIVWSVDI TAGGFCAALE

SLTNISSCNA IHRTQRILNG LCNQKASDVA SSPPDTKIEV AQFISKLLNY

SKQLFRYGH

For In vitro research Use Only. Not for use in or onhumans or animals or for diagnostics.  It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.


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