rev: October 20, 2004
Return (RDI HOME PAGE)
Return (mouse/rat cytokine index)
Murine/Rat Cytokine/Growth Factor/Chemokines Sample Spec sheets (subject to change without notice):
Rat Interleukin 4 is a potent lymphoid cell growth factor that stimulates
the growth and survivability of certain B cells and T cells. This Rat IL-4
is a 14kDa kDa protein containing 121 amino acid residues.
Catalog#: RDI-4004
Package Size: 10 micrograms $231.00/package $219.00/package 2+
Mini-Pack: cat#RDI-4004p $94.00/2ug Bulk quotes on request
Supplied: sterile filtered and Lyophilized protein, no carrier protein or
other additives.
Source: E. Coli
Purity: >98% by SDS-Page analysis and HPLC analysis. Endotoxin level is
less than 0.1ng per ug (1EU/ug) of rat IL-4.
Reconstitution: The lyophilized IL-4 is soluble in water and most aqueous
(ph >7) buffers. The lyophilized powder should be reconstituted
in H20 to a concnetration of 0.1-1.0mg/ml.. Allow to set at least
30 minutes at 4 DEG C , tighten cap and rotate to reconstitute any
material on sides of vial. If desired, you can centrifuge at 1000
rpm for 1 minute to concentrate volume in bottom of vial. This solution
can be diluted into water or other buffered solutions or stored at -20 DEG
C for future use.
Note:If this material is not to be used for coating or labeling (or other
applications requiring carrier free material), the addition of 0.1% BSA to
dilution buffers or dilution in sterile culture media is recommended to enhance
stability and minimize absorption to vial. Ideally, maintain stock
>10ug/ml.
Storage: The lyophilized powder, though stable at room temperature, is best
stored desiccated below 0 DEG C. Reconstituted rat IL-4 should be stored
in working aliquots at -20 DEG C. Avoid Frequent Freeze-THAW Cycles.
Reactivity: This material is fully biologically active when compared to
standards. The ED50 as determined by the dose dependent stimulation of the
proliferation of con A-activated rat spleen cells was found to be
<1.5ng/ml, yielding a specific activity of > 6.6 X 10 E06 units/mg
AA Sequence :
MHGCNDSPLR EIINTLNQVT EKGTPCTEMF VPDVLTATRN TTENELICRA
SRVLRKFYFP RDVPPCLKNK SGVLGELRKL CRGVSGLNSL RSCTVNESTL
TTLKDFLESL KSILRGKYLQ SCTSMS
Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.
Return (RDI HOME PAGE)
Return (mouse/rat cytokine index)
RDI Division of Fitzgerald Industries Intl
34 Junction Square Drive
Concord MA 01742-3049
USA
phone (978) 371-6446 or (800) 370-2222
fax (978) 371-2266
all rights reserved by any/all copyright holders?