rev: December 14, 2004
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Murine/Rat Cytokine/Growth Factor/Chemokines Sample Spec sheets (subject to change without notice):
IL-2 is a powerful immunoregulatory lymphokine produced by T-cells in response
to antigenic or mitogenic stimulation. IL-2/IL-2R signaling is required for
T-cell proliferation and other fundamental functions which are essential
for the immune response. IL-2 stimulates growth and differentiation of B-cells,
NK cells, lymphokine activated killer cells, monocytes, macrophages and
oligodendrocytes. Recombinant rat IL-2 is a 15.3 kDa protein containing 134
amino acid residues.
Package Size: 20 micrograms $231.00 $219.00/package 2 or more
-Mini-Pack# cat#RDI-4002p $94.00/5ug
Bulk quotes on request
Supplied: Lyophilized powder from 10mM sodium citrate pH 4.0 with no additives.
Source: E. Coli
Purity: >98% by SDS-Page and HPLC analysis. Endotoxin level is less than
0.1ng per ug
Reconstitution: The lyophilized rtIL2 should be reconstituted in water to a concentration of 0.1-1.0mg/ml. Allow to set at least 30 minutes at 4 DEG C, Mix well. If desired, centrifuge for 1-2 minutes at 1000 rpm to concentrate material in vial. This solution can be diluted into other buffered solutions or stored at 4 DEG C for 1 week or -20 DEG C for future use.
Note:If this material is not to be used for coating or labeling (or other applications requiring carrier free material), the addition of 0.1% BSA to dilution buffers or dilution in sterile culture media is recommended to enhance stability and minimize absorption to vial. Ideally, maintain stock >20ug/ml.
Storage: The lyophilized powder is stable at room temperature for a few week, but it is best stored desiccated at - 20 Deg C.Reconstituted rat IL-2 should be stored in working aliquots at -20 DEG C. AVOID FREQUENT FREEZE THAW CYCLES.
Reactivity: The ED50 as determined by the dose-dependent stimulation of the proliferation of murine CTLL-2 cells is < 0.4 ng/ml, corresponding to a specific activity of > 2.5 x 10 E06 units/mg.
AA Sequence: MAPTSSPAKE TQQHLEQLLL DLQVLLRGID NYKNLKLPMM LTFKFYLPKQ
ATELKHLQCL ENELGALQRV LDLTQSKSFH LEDAGNFISN IRVTVVKLKG
SENKFECQFD DEPATVVEFL RRWIAIAQSI ISTM
Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.
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