Murine/Rat Cytokine Data Sheets (murine sCD40L) available from Research Diagnostics Inc

rev: October 20, 2004

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MOUSE/RAT Cytokine/Growth Factor/Chemokines Sample Spec sheets (subject to change without notice):

DATA SHEET:recombinant MURINE soluble CD40Ligand/TRAP

Description: CD40L is a type II transmembrane glycoprotein belonging to the TNF superfamily of ligands. Naturally, CD40L is a membrane-bound protein, but proteolytically cleaved 15-18 kDa soluble forms of CD40L are also fully active. CD40L is a cell surface protein expressed on B cells, dendritic cells, monocytes, thymic epithelial cells and, at low levels, on T cells. Signaling through its receptor called CD40; CD40L plays an important role in the proliferation and differentiation of B cells, and is critical for immunoglobulin (Ig) class switching. The membrane anchored CD40L is expressed almost exclusively on activated CD4+ T lymphocytes. Failure to express CD40L leads to immunodeficiency with hyper-IgM, a disease characterized by failure to produce IgG, IgA and IgE. This recombinant Murine CD40L is a soluble 16.4 kDa protein containing 149 amino acid residues.

Catalog#: RDI-3515

Package Size: 25 micrograms $250.00 $219.00/package 2+

Mini-Pack: cat#RDI-3515p $94.00/5ug Bulk quotes on request

Supplied: Sterile filtered solution of 10mM sodium Phosphate pH7.5 & 0.1M arginine, & lyophilized (no carrier)

Source: E. Coli

Purity: >98% by SDS-Page and HPLC analysis Endotoxin level is less than 0.1ng per ug (1EU/ug) of sCD40L/TRAP.

Reconstitution: We recommend a quick spin followed by reconstitution in water to a concentration of 0.1-1.0 mg/ml. Allow to set at least 30 minutes at 4 DEG C, tighten cap and rotate to reconstitute any material on sides of vial. If desired, you can centrifuge at 1000 rpm for 1 minute to concentrate volume in bottom of vial.This solution can be diluted into other buffered solutions containing carrier protein or medium and stored at 4 DEG C for up to 1 week or at -20 DEG C for future use.

Note:If this material is not to be used for coating or labeling (or other applications requiring carrier free material), the addition of 0.1% BSA to dilution buffers or dilution in sterile culture media is recommended to enhance stability and minimize absorption to vial. Ideally, maintain stock >10ug/ml.

Storage: The lyophilized powder is stable for a few weeks at room temperature but it is best stored desiccated at -20 DEG C. Reconstituted sCD40L should be stored in working aliquots at -20DEG C. AVOID FREQUENT FREEZE THAW CYCLES.

Reactivity: Determined by its abaility to induce TNF-Alpha and MIP- 1 alpha production by murine splenecytes. The expcetd ED50 for this effect is <0.1ug/ml

Sequence:

MQRGDEDPQI AAHVVSEANS NAASVLQWAK

KGYYTMKSNL VMLENGKQLT VKREGLYYVY

TQVTFCSNRE PSSQRPFIVG LWLKPSSGSE

RILLKAANTH SSSQLCEQQS VHLGGVFELQ

AGASVFVNVT EASQVIHRVC FSSFGLLKL

Precautions:For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.