Human Cytokine Data Sheets (Recombinant APO-E) available through Research Diagnostics Inc

rev: November 9, 2004

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Human Cytokine/Growth Factor/Chemokines Sample Spec sheets (subject to change without notice):
Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.

DATA SHEET:recombinant Human ApoE

-see 3 forms blelow APO-E2, APO-E3 and ApoE4

DATA SHEET:recombinant Human ApoE2

-ApoE belongs to a group of proteins that bind reversibly with lipoprotein and play an important role in lipid metabolism. In addition to facilitating solublization of lipids, these proteins help to maintain the structural integrity of lipoproteins, serve as ligands for lipoprotein receptors, and regulate the activity of enzymes involved in lipid metabolism. Significant quantities of ApoE are produced in liver and brain and to some extent in almost every organ. ApoE is an important constituent of all plasma lipoproteins. It’s interaction with specific ApoE receptor enables uptake of chylomicron remnants by liver cells, which is an essential step during normal lipid metabolism. It also binds with the LDL receptor (apo B/E). Defects in ApoE are a cause of hyperlipoproteinemia type III. ApoE exists in three major isoforms; E2, E3, and E4, which differ from one another by a single amino-acid substitution. Compared with E3 and E4, E2 exhibits the lowest receptor binding affinity. E2 allele carriers had significantly lower levels of total cholesterol, low-density lipoprotein cholesterol, and non-high-density lipoprotein cholesterol, as well as increased ApoE levels. This recombinant human ApoE2 is a 34.3 kDa protein containing 300 amino acid residues.

Catalog#: RDI-3512

Package Size: 500 micrograms $235.00 $219.00/vial 2+

Supplied: Lyophilized powder from 20mM Sodium Phosphate, pH7.8 + 0.5mM DTT.

Source: E. Coli

Purity: >90% by SDS-Page and HPLC analysis. Endotoxin level is less than 0.1ng per ug (1EU/ug) of ApoE2.

Reconstitution: Lyophilized ApoE should be reconstituted in water to a concentration of 0.1-1mg/ml. Allow to set at least 30 minute sat 4 DEG C, Mix Well. If desired, centrifuge for 1-2 minutes at 1000 rpm to concentrate material in vial. This solution can be diluted into other buffered solutions or stored at -w0 DEG C for future use.

Note:If suitable for use (ie, if this material is not being used labelling), we recommned adding 0.1% protease free BSA to dilution buffers to minimize absorption on containers.

Storage: The lyophilized powder is stable at room temperature but it is best stored desiccated below 0 Deg C. Reconstituted ApoE should be stored in working aliquots at -20 DEG C. AVOID FREQUENT FREEZE THAW CYCLES.

Reactivity: in testing

Sequence:

MKVEQAVETE PEPELRQQTE WQSGQRWELA LGRFWDYLRW VQTLSEQVQE

ELLSSQVTQE LRALMDETMK ELKAYKSELE EQLTPVAEET RARLSKELQA

AQARLGADME DVCGRLVQYR GEVQAMLGQS TEELRVRLAS HLRKLRKRLL

RDADDLQKCL AVYQAGAREG AERGLSAIRE RLGPLVEQGR VRAATVGSLA

GQPLQERAQA WGERLRARME EMGSRTRDRL DEVKEQVAEV RAKLEEQAQQ

IRLQAEAFQA RLKSWFEPLV EDMQRQWAGL VEKVQAAVGT SAAPVPSDNH

Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.

-Recombinant APOE3-

ApoE3 belongs to a group of proteins that bind reversibly with lipoprotein and play an important role in lipid metabolism. In addition to facilitating solublization of lipids, these proteins help to maintain the structural integrity of lipoproteins, serve as ligands for lipoprotein receptors, and regulate the activity of enzymes involved in lipid metabolism. Significant quantities of ApoE are produced in liver and brain and to some extent in almost every organ. ApoE exists in three major isoforms; E2, E3, and E4, which differ from one another by a single amino-acid substitution. E3 is the most common isoform and is present in 40-90% of the population. This recombinant human ApoE3 is a 34.0 kDa protein containing 299 amino acid residues

Catalog#: RDI-3502

Package Size: 500 micrograms $312.00 $219.00/vial 2+

Supplied: Lyophilized powder from 20mM Sodium Phosphate, pH7.8 + 0.5mM DTT.

Source: E. Coli

Purity: >90% by SDS-Page and HPLC analysis. Endotoxin level is less than 0.1ng per ug (1EU/ug) of ApoE.

Note:If suitable for use (ie, if this material is not being used labelling), we recommned adding 0.1% protease free BSA to dilution buffers to minimize absorption on containers.

Reactivity: in testing

Sequence:

MKVEQAVETE PEPELRQQTE WQSGQRWELA LGRFWDYLRW VQTLSEQVQE

ELLSSQVTQE LRALMDETMK ELKAYKSELE EQLTPVAEET RARLSKELQA

AQARLGADME DVCGRLVQYR GEVQAMLGQS TEELRVRLAS HLRKLRKRLL

RDADDLQKRL AVYQAGAREG AERGLSAIRE RLGPLVEQGR VRAATVGSLA

GQPLQERAQA WGERLRARME EMGSRTRDRL DEVKEQVAEV RAKLEEQAQQ

IRLQAEAFQA RLKSWFEPLV EDMQRQWAGL VEKVQAAVGT SAAPVPSDNH

It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.

DATA SHEET: recombinant Human ApoE4

ApoE belongs to a group of proteins that bind reversibly with lipoprotein and play an important role in lipid metabolism. In addition to facilitating solublization of lipids, these proteins help to maintain the structural integrity of lipoproteins, serve as ligands for lipoprotein receptors, and regulate the activity of enzymes involved in lipid metabolism. Significant quantities of ApoE are produced in liver and brain and to some extent in almost every organ. ApoE exists in three major isoforms; E2, E3, and E4, which differ from one another by a single amino-acid substitution. Individuals heterozygous for the ApoE4 allele are at high risk of late-onset Alzheimer's disease. This recombinant human ApoE4 is a 34.4 kDa protein containing 300 amino acid residues.

Catalog#: RDI-3504

Package Size: 500 micrograms $250.00 $219.00/vial 2+

Supplied: Lyophilized powder with no additives

Source: E. Coli

Purity: >90% by SDS-Page and HPLC analysis. Endotoxin level is less than 0.1ng per ug (1EU/ug) of ApoE.

Note:If suitable for use (ie, if this material is not being used labelling), we recommned adding 0.1% protease free BSA to dilution buffers to minimize absorption on containers.

Reactivity: in testing

AA Sequence :

MKVEQAVETE PEPELRQQTE WQSGQRWELA LGRFWDYLRW VQTLSEQVQE ELLSSQVTQE LRALMDETMK ELKAYKSELE EQLTPVAEET RARLSKELQA AQARLGADME DVRGRLVQYR GEVQAMLGQS TEELRVRLAS HLRKLRKRLL RDADDLQKRL AVYQAGAREG AERGLSAIRE RLGPLVEQGR VRAATVGSLA GQPLQERAQA WGERLRARME EMGSRTRDRL DEVKEQVAEV RAKLEEQAQQ IRLQAEAFQA RLKSWFEPLV EDMQRQWAGL VEKVQAAVGT SAAPVPSDNH