rev: November 18, 2004
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Murine/Rat Cytokine/Growth Factor/Chemokines Sample Spec sheets (subject to change without notice):
see also rat GM-CSF and human GM-CSF
DATA SHEET:recombinant MURINE GRANULOCYTE MACROPHAGE COLONY STIMULATING FACTOR
GM-CSF is a hematopoietic growth factor that stimulates the development of
neutrophils and macrophages and promotes the proliferation and development
of early erythroid megakaryocytic and eosinophilic progenitor cells. It is
produced in by endothelial cells, monocytes, fibroblasts and T-lymphocytes.
GM-CSF inhibits neutrophil migration and enhances the functional activity
of the mature end-cells. The human and murine molecules are species-specific
and exhibit no cross-species reactivity. This recombinant murine GM-CSF is
a 14.2 kDa globular protein consisting of 124 amino acids residues
Catalog#: RDI-3153
Batch# $231.00/package $219.00/package 2+
Mini-Pack:cat#RDI-3153p $94.00/2ug
bulk quotes on request email: cytokines@researchd.com
Bulk quotes on request
Package Size: 10 micrograms
Supplied: Lyophilized protein with no additives.
Source: E. Coli
Purity: >98% by SDS-Page analysis. Endotoxin level is less than 0.1ng/ug
(1EU/ug) of murine GM-CSF.
Reconstitution: The lyophilized GM-CSF is soluble in water and most aqueous
buffers. The lyophilized protein can be reconstituted in water to a concentration
of 100ng/ul. Allow to set at least 30 minutes at 4 DEG C , tighten cap and
rotate to reconstitute any material on sides of vial. If desired, you can
centrifuge at 1000 rpm for 1 minute to concentrate volume in bottom of vial.
This solution can be diluted into water or other buffered solutions or stored
at 4 DEG C for up to 1 week or at -20 DEG C for future use. For most in-vitro
applications, murine GM-CSF exerts its biological activity in the concentration
range of 0.05 to 0.5ng/ml.
Note:If this material is not to be used for coating or labeling (or other
applications requiring carrier free material), the addition of 0.1% BSA or
dilution in sterile culture media is recommended to enhance stability and
minimize absorption to vial.
Storage: The lyophilized powder, though stable at room temperature for a
few weeks, is best stored dessicated dessicated at -20 DEG C. Reconstituted
GM-CSF should be stored in working aliquots at -20 DEG C. Avoid Frequent
Freeze-THAW Cycles.
Reactivity: This product is fully biologically active when compared to standards.
The Ed50 as determined by the dose dependent stimulation of the proliferation
of murine FDC-P1 cells is <0.2ng/ml, which yields a specific activity
of >5 million units/mg.
AA Sequence:
MAPTRSPITV TRPWKHVEAI KEALNLLDDM PVTLNEEVEV
VSNEFSFKKL TCVQTRLKIF EQGLRGNFTK LKGALNMTAS
YYQTYCPPTP ETDCETQVTT YADFIDSLKT FLTDIPFECK KPVQK
Precautions: For In vitro research Use Only. Not for use in or on humans
or animals or for diagnostics. It is the responsibility of the user to comply
with all local/state and Federal rules in the use of this product. We are
not responsible for any patent infringements that might result with the use
of or derivation of this product.
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