rev: November 18, 2004
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Murine/Rat Cytokine/Growth Factor/Chemokines Sample Spec sheets (subject to change without notice):
DATA SHEET:recombinant MURINE MACROPHAGE COLONY STIMULATING FACTOR
M-CSF is a potent hematopoietic factor produced by a variety of cells including lymphocytes, monocytes, fibroblasts, endothelial cells, myoblasts and osteoblasts. It is a key regulator of cellular proliferation, differentiation, and survival of blood monocytes, tissue macrophages and their progenitor cells. M-CSF has been shown to play important roles in modulating dermal thickness, and male and female fertility. M-CSF is clinically used in the treatment of infection, malignancies and atherosclerosis. It facilitates hematopoietic recovery after bone marrow transplantation. The human is reactive in murine systems, but the murine molecule exhibits no activity on human cells. This recombinant murine M-CSF is a 36.4 kDa homodimeric protein containing two 156 amino acid polypeptide subunits.
Catalog#: RDI-3152
Package: $231.00/package $219.00/package 2+
Mini-Pack:cat#RDI-3152p $94.00/2ug Bulk quotes on request
Package Size: 10 micrograms
Supplied: Lyophilized protein from 5mm Tris,pH8.0 + 100mM NaCl. no
additive/carrier.
Source: E. Coli
Purity: >98% by SDS-Page analysis. Endotoxin level is less than 0.1ng/ug
(1EU/ug) of murine M-CSF.
Reconstitution: First tighten cap and gently tap on table or centrifuge 10 seconds 1000RPM to settle contents in vial. The lyophilized G-CSF is soluble in water and most aqueous buffers. The lyophilized protein SHOULD be reconstituted in water to a concentration of 0.1-1mg/ml. Allow to set at least 30 minutes at 4 DEG C , tighten cap and rotate to reconstitute any material on sides of vial. If desired, you can centrifuge at 1000 rpm for 1 minute to concentrate volume in bottom of vial. This solution can be diluted into other buffered solutions or stored at 4 DEGC for up to 1 week or at -20 DEG C for future use.
Note:If this material is not to be used for coating or labeling (or other applications requiring carrier free material), the addition of 0.1% BSA or dilution in sterile culture media is recommended to enhance stability and minimize absorption to vial.
Storage: The lyophilized powder, though stable at room temperature for a
few weeks, is best stored dessicated dessicated at -20 DEG C. Reconstituted
M-CSF should be stored in working aliquots at -20 DEG C. Avoid Frequent
Freeze-THAW Cycles.
Reactivity: The Ed50 as determined by the dose dependent stimulation of the
proliferation of M-NFS-60 cells is <1ng/ml,which yields a specific activity
of >1 million units/mg.
AA Sequence:
MKEVSEHCSH MIGNGHLKVL QQLIDSQMET
SCQIAFEFVD QEDLDDPVCY LKKAFFLVQD
IIDETMRFKD NTPNANATER LQELSNNLNS
CFTKDYEEQN KACVRTFHET PLQLLEKIKN
FFNETKNLLE KDWNIFTKNC NNSFAKCSSR
DVVTKP
Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.
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