rev:    October 20, 2004

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Murine/Rat Cytokine/Growth Factor/Chemokines Sample Spec sheets (subject to change without notice):


DATA SHEET:recombinant MURINE Thrombopoietin

-MURINE TPO ( Thrombopoietin, Mpl-ligand, MGDF) stimulates the proliferation and maturation of megakaryocytes and promotes increased circulating levels of platelets in vivo. This recombinant murine TPO is a soluble 18.7 kDa protein containing 174 amino acid residues which comprises the receptor binding domain of the Mpl-ligand protein.

Catalog#: RDI-3114

Package Size: 10 micrograms $231.00/package   $219.00/package 2+

                    mini-pack RDI-3114P $94.00/2ug    Custom & Bulk on request


Supplied: Lyophilized powder from sterile filtered 10mM Tris pH8.0

Source: E. Coli

Purity: >98% by SDS-Page analysis. Endotoxin level is less than 0.1ng per ug of   TPO.

Reconstitution: The lyophilized TPO should be reconstituted in 10mM Tris pH 8 or distilled water to a concentration of 0.1-1.0 milligram/ml . Allow to set at 4 DEG C for at least 30 minutes, tighten cap, mix well. If desired, centrifuge for 1-2 minutes at 1000 rpm to concentrate material in vial. This solution should be diluted into buffer containing carrier protein or medium containing serum and stored at 4 DEG C for up to 1 week or at -20 DEG C for long term use.

Storage: The lyophilized powder is stable at room temperature for a few weeks but it is best stored desiccated at -20 Deg C. Reconstituted material should be stored at 4 DEG C or in working aliquots at -20 DEG C. AVOID FREQUENT FREEZE THAW CYCLES.

Reactivity: The ED50 as determined by the dose dependent stimulation of human M07e cells was found to be < 1.0ng/ml corresponding to a specific activity of > 1 million units/mg.

AA Sequence:

SPVAPACDPR LLNKLLRDSH LLHSRLSQCP DVDPLSIPVL

LPAVDFSLGE WKTQTEQSKA QDILGAVSLL LEGVMAARGQ

LEPSCLSSLL GQLSGQVRLL LGALQGLLGT QLPLQGRTTA

HKDPNALFLS LQQLLRGKVR FLLLVEGPTL CVRRTLPTTA

VPSSTSQLLT LNKF

Precautions: For In vitro research Use Only. Not for use   in or on humans or animals or for diagnostics.  It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.


see also human TPO

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