rev: February 2, 2005
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MURINE Cytokine/Growth Factor/Chemokines Sample Spec sheets (subject to change without notice):
-RANKL and RANK are members of the TNF superfamily of ligands and receptors
that play an important role in the regulation of specific immunity and bone
turnover. RANK (receptor) was originally identified as a dendritic-cell-membrane
protein, which by interacting with RANKL augments the ability of dendritic
cells to stimulate naïve T-cell proliferation in a mixed lymphocyte
reaction, to promote the survival of RANK + T cells, and to regulate
T-cell-dependent immune response. RANKL, which is expressed in a variety
of cells including osteoblasts, fibroblasts, activated T-cells and bone marrow
stromal cells, is also capable of interacting with a decoy receptor called
OPG. Binding of soluble OPG to sRANKL inhibits osteoclastogenesis by interrupting
the signaling between stromal cells and osteoclastic progenitor cells, thereby
leading to excess accumulation of bone and cartilage. This recombinant murine
sRANKL is a 19.4 kDa polypeptide comprising the TNF homologous region of
RANKL (174 amino acid residues.
Catalog#: RDI-3111
Package Size: 10 micrograms $231.00 $219.00/package 2+
Mini-Pack cat#RDI-3111P $94.00 inquire for bulk
Supplied: Sterile filtered & lyophilized from 5mM Tris pH7.6 + 75mM NaCl.
No carrier.
Source: E. Coli
Purity: >98% by SDS-Page and HPLC analysis Endotoxin level is less than
0.1ng per ug (1EU/ug) of RANKL.
Reconstitution: The lyophilized RANKL is soluble in most aqueous buffers.
The lyophilized protein should be reconstituted to a concentration of 0.1-1mg/ml.
Allow to set at least 30 minutes at 4 DEG C, tighten cap and rotate to
reconstitute any material on sides of vial. If desired, you can centrifuge
at 1000 rpm for 1 minute to concentrate volume in bottom of vial. This solution
can be diluted into other buffered solutions containing carrier protein or
medium and stored at 4 DEG C for up to 1 week or at -20 DEG C for future
use.
Note:If this material is not to be used for coating or labeling (or other applications requiring carrier free material), the addition of 0.1% BSA to dilution buffers or dilution in sterile culture media is recommended to enhance stability and minimize absorption to vial. Ideally, maintain stock >10ug/ml.
Storage: The lyophilized powder is stable at room temperature for a few weeks but it is best stored desiccated at -20 Deg C. Reconstituted RaNKL should be stored in working aliquots at -20DEG C. AVOID FREQUENT FREEZE THAW CYCLES.
Reactivity: Measured by it's ability to induce osteoclast formation on murine RAW264.7 cells using a concentration of 5-10ng.ml.
AA Sequence :
MAPAMMEGSW LDVAQRGKPE AQPFAHLTIN AASIPSGSHK
VTLSSWYHDR GWAKISNMTL SNGKLRVNQD GFYYLYANIC
FRHHETSGSV PTDYLQLMVY VVKTSIKIPS SHNLMKGGST
KNWSGNSEFH FYSINVGGFF KLRAGEEISI QVSNPSLLDP
DQDATYFGAF KVQDID
Precautions:For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.
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