rev: November 2, 2004
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MOUSE/RAT Cytokine/Growth Factor/Chemokines Sample Spec sheets (subject to change without notice):
DATA SHEET:recombinant MURINE TUMOR NECROSIS FACTOR-A
-TNF-alpha is a pleiotrophic pro-inflammatory cytokine secreted by various cells including adipocytes, activated monocytes, macrophages, B cells, T cells and fibroblasts. It belongs to the TNF family of ligands and signals through two receptors, TNFR1 and TNFR2. TNF-alpha is cytotoxic to a wide variety of tumor cells and is an essential factor in mediating the immune response against bacterial infections. TNF-alpha also plays a role in the induction of septic shock, auto immune diseases, rheumatoid arthritis, inflammation, and diabetes. This recombinant murine TNF-alpha is a soluble 156 amino acid protein (17.3 kDa) which corresponds to C-terminal extracellular domain of the full length transmembrane protein.
Catalog#: RDI-3101a
Package Size: 20 micrograms $235.00 $219.00/PACKAGE 2+
Mini-Pack:cat#RDI-3101ap $94.00/5ug inquire for bulk
Supplied: Lyophilized powder from 5mM Tris pH 8.0 with no additives.
Source: E. Coli
Purity: >98% by SDS-Page analysis and HPLC analysis . Endotoxin level
is less than 0.1ng per ug of murine TNF-A
Reconstitution: The lyophilized TNF-A should be reconstituted in sterile
water to a concentration of 0.1-1mg/ml. Allow to set at least 30 minutes
at 4 DEG C, rotate/mix well. This solution can be diluted into other buffered
solutions or stored at 4 DEG C for up to 1 week or at -20 DEG C for future
use. For most in-vitro applications, TNF-A exerts its biological activity
in the range of 0.05 to 20.0 ng/ml.
Note:Note:If this material is not to be used for coating or labeling (or
other applications requiring carrier free material), the addition of 0.1%
BSA to dilution buffers or dilution in sterile culture media is recommended
to enhance stability and minimize absorption to vial.
Storage: The lyophilized powder is stable at room temperature for a few weeks
but it is best stored desiccated at -20 DEG C. Reconstituted TNF-A should
be stored in working aliquots at -20DEG C. AVOID FREQUENT FREEZE THAW
CYCLES.
Reactivity: The ED50 as determined by the cytolysis of murine L929 cells
in the presence of Actinomycin-D is less than 0.1 ng/ml. This yields a specific
activity of greater than 1 X 107 units/mg.
AA Sequence:
MLRSSSQNSS DKPVAHVVAN HQVEEQLEWL SQRANALLAN
GMDLKDNQLV VPADGLYLVY SQVLFKGQGC PDYVLLTHTV
SRFAISYQEK VNLLSAVKSP CPKDTPEGAE LKPWYEPIYL
GGVFQLEKGD QLSAEVNLPK YLDFAESGQV YFGVIAL
Precautions: For In vitro research Use Only. Not for use in or on humans
or animals or for diagnostics. It is the responsibility of the user
to comply with all local/state and Federal rules in the use of this product.
We are not responsible for any patent infringements that might result with
the use of or derivation of this product.
Precautions:
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