rev: October 19, 2004
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Human Cytokine/Growth Factor/Chemokines Sample Spec sheets (subject to change without notice):
-Recombinant Human beta-defensin-3 (BD-3) in an antimicrobial peptide, which belongs to the distinct family of beta-defenisns. Recombinant Human BD-3 is a 5.1 kDa protein containing 45 amino acid residues.
Catalog#: RDI-3052
Package Size: 20 micrograms $231.00 $219.00/pack 2+
Mini-Pack: cat#RDI-3052P $94.00/5ug
Supplied: Lyophilized powder from 10 mM Acetic Acid with no additives.
Source: E. Coli
Purity: >95% by SDS-Page analysis. Endotoxin level is less than 0.1ng
per ug (1EU/ug) of protein
Reconstitution: Lyophilized BD-23should be reconstituted in 10mM acetic acid
to a concentration of 0.1-1mg/ml. Allow to set at least 30 minutes at 4 DEG
C, mix well. If desired, tighten cap and centrifuge for 1 minute at 1000rpm
to concentrate material in vial. This solution can be diluted into other
buffered solutions or stored at 4 DEG C for up to 1 week or at -20 DEG C
for future use. -Note:If this material is not to be used for coating or labeling
(or other applications requiring carrier free material), the addition of
0.1% BSA or HSA to dilution buffers or dilution in sterile culture media
is recommended to enhance stability and minimize absorption to vial. Ideally,
maintain stock >20ug/ml.
Storage: The lyophilized powder is stable at room temperature for a few weeks
but it is best stored desiccated at -20 DEG C. Reconstituted BD- 3should
be stored in working aliquots at -20 DEG C. AVOID FREQUENT FREEZE THAW
CYCLES.
Biological Activity : Exhibits antimicrobial activity against gram-positive
bacteria S. aureus and gram-negative P. aeruginosa and E.coli.
AA Sequence : GIINTLQKYY CRVRGGRCAV LSCLPKEEQI GKCSTRGRKC CRRKK
Precautions: For In vitro research Use Only. Not for use in or on humans
or animals or for diagnostics. It is the responsibility of the user
to comply with all local/state and Federal rules in the use of this product.
We are not responsible for any patent infringements that might result with
the use of or derivation of this product.
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Concord MA 01742-3049
USA
phone (978) 371-6446 or (800) 370-2222
fax (978) 371-2266
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