rev: Ocotber 19, 2004
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Human Cytokine/Growth Factor/Chemokines Sample Spec sheets (subject to change without notice):
Human BRAK is a newly discovered CXC chemokine, BRAK shares 30% identity with MIP-2 alpha and beta and shares several biological activities. This human BRAK is an 9.4kDa protein containing 77 amino acid residues.
Catalog#: RDI-3050
Package Size: 20 micrograms $231.00 $219.00/pack 2+
Mini-pack:cat#RDI-3050p $94.00/5ug
Supplied: sterile filtered,lyophilized protein with no additives
Source: E. Coli
Purity: >98% by SDS-Page analysis. Endotoxin level is less than 0.1ng
per ug (1EU/ug).
Reconstitution: The lyophilized protein soluble in water and most aqueous
buffers. The lyophilized powder should first be quick spun in vial, then
reconstituted in water to a concentration of 0.1-1.0mg/ml. Allow to set at
least 30 minutes at 4 DEG C. If desired, can centrifuge for 1 minute at 1000
rpm to concentrate material in vial. This solution can be diluted into water
or other buffered solutions or stored at 4 DEG C for up to 1 week or at -20
DEG C for future use.
Note:If this material is not to be used for coating or labeling (or other applications requiring carrier free material), the addition of 0.1% BSA to dilution buffers or dilution in sterile culture media is recommended to enhance stability and minimize absorption to vial. Ideally, maintain stock >10ug/ml.
Storage: The lyophilized powder, though stable at room temperature for a few weeks, is best stored dessicated at -20 DEG C. Reconstituted BRAK should be stored in working aliquots at -20 DEG C. Avoid Frequent Freeze-THAW Cycles.
Biological Activity: determined by its ability to chemoattract activated monocytes using a concentration range of 1.0-10.0ng/ml.
AA Sequence :
SKCKCSRKGP KIRYSDVKKL EMKPKYPHCE
EKMVIITTKS VSRYRGQEHC LHPKLQSTKR
FIKWYNAWNE KRRVYEE
Precautions: For In vitro research Use Only. Not for use in or on humans
or animals or for diagnostics. It is the responsibility of the user
to comply with all local/state and Federal rules in the use of this product.
We are not responsible for any patent infringements that might result with
the use of or derivation of this product.
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phone (978) 371-6446 or (800) 370-2222
fax (978) 371-2266
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