rev:   Ocotber 19, 2004


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Human Cytokine/Growth Factor/Chemokines Sample Spec sheets (subject to change without notice):


-Human I-TAC (Interferon-inducible T cell alpha chemoattractant) is a novel non-ELR CXC chemokine. Human I-TAC is regulated by interferon and has potent chemoattractant activity for interleukin-2 activated T cells, but not for freshly isolated unstimulated T cells, neutrophils or monocytes. This rec human I-TAC is a 8.3 kDa protein containing 73 amino acid residues.

Catalog#: RDI-3046

Package Size: 20 micrograms $231.00     $219.00/package 2+

                         Mini-Pack: cat#RDI-3046P $94.00/5ug    inquire for bulk

Supplied: Lyophilized powder with no additives.

Source: E. Coli

Purity: >98% by SDS-Page and HPLC analysis Endotoxin level is less than 0.1ng per ug (1EU/ug) of I-TAC.

Reconstitution: The lyophilized I-Tac should be reconstituted in water to a concentration of 0.1- 1.0mg/ml. Allow to set at least 30 minutes at 4 DEG C, tighten cap and rotate to reconstitute any material on sides of vial. If desired, you can centrifuge at 1000 rpm for 1 minute to concentrate volume in bottom of vial. This solution can be diluted into other buffered solutions containing carrier protein or medium and stored at 4 DEG C for up to 1 week or long term at -20 DEG C for future use.

Note:If this material is not to be used for coating or labeling (or other applications requiring carrier free material), the addition of 0.1% BSA to dilution buffers or dilution in sterile culture media is recommended to enhance stability and minimize absorption to vial. Ideally, maintain stock >50ug/ml.

Storage: The lyophilized powder is stable at room temperature for a few weeks but it is best stored desiccated at -20 DEG C. Reconstituted I-TAC should be stored in working aliquots at -20DEG C. AVOID FREQUENT FREEZE THAW CYCLES.

Reactivity: The reactivity of this rhuman I-TAC was determined by a chemotaxis assay using IL-2 activated human T cells. Significant chemotaxis was achieved using a concentration of 0.1-10ng/ml.

AA Sequence:



Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.


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