rev:    October 19, 2004


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Human Cytokine/Growth Factor/Chemokines Sample Spec sheets (subject to change without notice):

recombinant Human Hepatocellular Carcinoma (HCC-1)

-Human Hepatocellular Carcinoma (HCC-1) was first isolated from the hemofiltrate of patients with chronic renal failure. HCC-1 has a 46% amino acid sequence identity with MIP-1 alpha/MIP-1Beta and 29%-37% homology with other CC chemokines. This recombinant Human HCC-1 is an 8.4 kda protein containing 72 amino acid residues.

Catalog#: RDI-3038

Package Size: 10 micrograms $231.00    $219.00/pack 2 or more  other sizes on request

                      MINI-PACK cat#RDI-3038p $94.00/2ug

Purity: >98% by SDS-Page and HPLC analyses. Endotoxin level is   less than 0.1ng per ug (1EU/ug) of HCC-1

Reconstitution: The lyophilized human HCC-1 is soluble in water and most aqueous buffers. The material can be reconstituted in water to a concentration of 0.1- 1mg/ml. Allow to set at least 30 minutes at 4 DEG C, tighten cap and rotate to reconstitute any material on sides of vial. If desired, you can centrifuge at 1000 rpm for 1 minute to concentrate volume in bottom of vial. This solution can be diluted into water or other buffered solutions or stored at 4 DEG C for future use. Avoid freeze thaw cycles which will result in some loss of activity.

Note:If this material is not to be used for coating or labeling(or other applications requiring carrier free material), the addition of 0.1% BSA to dilution buffers or dilution in sterile culture media is recommended to enhance stability and minimize absorption to vial. Ideally, maintain concentration >10ug/ml.

Storage: The lyophilized powder is stable at room temperature for a few weeks but it is best stored desiccated at -20 DGE C. Reconstituted human HCC-1 should be stored in working aliquots at -20 DEG C. AVOID FREQUENT FREEZE THAW CYCLES. EACh FREEZE THAW CYCLE WILL DIMINISH ACTIVITY.

Reactivity: Determined by its ability to chemoattract human monocytes using a concnetration range of 5-20.0ng/ml.

AA Sequence:



For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.  It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.

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phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

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