rev:    October 19, 2004


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Human Cytokine/Growth Factor/Chemokines Sample Spec sheets (subject to change without notice):

DATA SHEET: recombinant HUMAN I-309

-Human I-309 is a small glycoprotein secreted by activated T lymphocytes and structurally related to other CC chemokines. This rec human I-309 is an 8.5kDa protein containing 74 amino acid residues.

Catalog#: RDI-3037

Package Size: 10 micrograms $231.00     $219.00/vial 2 or more

                        mini-pack cat#RDI-3037P $94.00/2ug

                    -Bulk and other sizes Quotes upon request

Supplied: Lyophilized powder from sterile filtered PBS with no additives.

Source: E. Coli

Purity: >98% by SDS-Page and HPLC analysis Endotoxin level is less than 0.1ng per ug (1EU/UG) of I-309

Reconstitution: The lyophilized I-309 is soluble in water and most aqueous bufers. It should be reconstituted to a concentration of 0.1-1.0mg/ml. Allow to set at least 30 minutes at 4 DEG C, tighten cap and rotate to reconstitute any material on sides of vial. If desired, you can centrifuge at 1000 rpm for 1 minute to concentrate volume in bottom of vial. This solution can be diluted into other buffered solutions containing carrier protein or medium and stored at 4 DEG C for up to 1 week or stored at -20 DEG C for future use.

Storage: The lyophilized powder is stable at room temperature for a few weeks but it is best stored desiccated at -20 Deg C. Reconstituted I-309 should be stored in working aliquots at -20DEG C. AVOID FREQUENT FREEZE THAW CYCLES.

Reactivity: The Biological activity of human I-309 was determined by a chemotaxis assay using human T cell populations. Significant chemotaxis was achieved at a concentration range of 10-100ng/ml

AA Sequence :



Precautions: For In vitro research Use Only. Not for use in  or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.


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