rev: October 19, 2004
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Human Cytokine/Growth Factor/Chemokines Sample Spec sheets (subject to change without notice):
-Human Cardiotrophin-1 is a member of the leukemia inhibitory/ciliary
neurotrophic factor/Oncostatin M/interleukin6/interleukin-11 family of cytokines.
Cardiotrophin-1 affects cells through interaction with the gp130 receptor
subunit. This recombinant version is a peptide of 21.5 kDa that consists
of 201 amino acid residues.
Catalog#: RDI-3032
Package Size: 10 micrograms $231.00 $219.00/package 2+
Mini-Pack: cat#RDI-3032P $94.00/2ug
-Bulk Quotes upon request
Supplied: Lyophilized powder from sterile filtered PBS with no additives.
Source: E. Coli
Purity: >98% by SDS-Page and HPLC analysis Endotoxin level is less than
0.1ng per ug (1EU/UG) of cariotrophin-1
Reconstitution: The lyophilized cardiotrophin-1 MUST be reconstituted in
20mM Tris , pH 8.0 (<1mg/ml). Allow to set at least 30 minutes at 4 DEG
C, tighten cap and rotate to reconstitute any material on sides of vial.
If desired, you can centrifuge at 1000 rpm for 1 minute to concentrate volume
in bottom of vial. This solution can be diluted into other buffered solutions
containing carrier protein or medium and stored at 4 DEG C for up to 1 week
or at -20 DEG C for future use. Cardiotrophin-1 exerts its biological activity
in the concentration of 0.1 to 10ng/ml.
Stability: The lyophilized protein is stable for a few weeks at room temperature,
but is best store dessicated at -20 DEG C. Reconstituted cardiotrophin-1
should be stored in working aliquots at -20 DEG C.
BiologicalActivity: The ED50 as determined by the dose dependent proliferation of TF-1 cell is <1ng/ml, correspondimg to a specific activity of > 1million units/mg.
AA Sequence:
MSRREGSLED PQTDSSVSLL PHLEAKIRQT HSLAHLLTKY AEQLLQEYVQ
LQGDPFGLPS FSPPRLPVAG LSAPAPSHAG LPVHERLRLD AAALAALPPL
LDAVCRRQAE LNPRAPRLLR RLEDAARQAR ALGAAVEALL AALGAANRGP
RAEPPAATAS AASATGVFPA KVLGLRVCGL YREWLSRTEG DLGQLLPGGS
A
Precautions: For In vitro research Use Only. Not for use in or on humans
or animals or for diagnostics. It is the responsibility of the user to comply
with all local/state and Federal rules in the useof this product. We are
not responsible for any patent infringements that might result with the use
of or derivation of this product.
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