rev:     October 19, 2004


Return (human cytokine index)

Human Cytokine/Growth Factor/Chemokines Sample Spec sheets (subject to change without notice):

DATA SHEET:recombinant Human MIP-3

-Human macrophage inflammatory protein-3 also called MPIF-1 is a member of the CC chemokine family of cytokines. It displays chemotactic activity on resting T lymphocytes and monocytes. Human MIP-3 is an 11.3kDa protein containing 99 amino acids residues.

Catalog#: RDI-3029C

Package Size: 20 micrograms $231.00    $219.00/pack 2 or more

                    MINI-PACK cat#RDI-3029Cp $94.00/2ug   inquire for bulk

Supplied: sterile filtered and then the protein was lyophilized (no additives)

Source: E. Coli

Purity: >99% by SDS-Page and HPLC analyses. Endotoxin level is less than 0.1ng per ug (1EU/ug) of MIP-3

Reconstitution: The lyophilized human MIP-3 is soluble in water and most aqueous buffers. The material can be reconstituted in water to a concentration of 1mg/ml.   Allow to set at least 30 minutes at 4 DEG C, tighten   cap and rotate to reconstitute any material on sides of vial. If desired, you can centrifuge at 1000 rpm for 1 minute to concentrate volume in bottom of vial. This solution can be diluted into water or other buffered solutions or stored at 4 DEG C for future use. Avoid freeze thaw cycles which will result in some loss of activity.

Note:If this material is not to be used for coating or labeling(or other applications requiring carrier free material), the addition of 0.1% BSA to dilution buffers or dilution in sterile culture media is recommended to enhance stability and minimize absorption to vial. Ideally, maintain concentration >50ug/ml.

Storage: The lyophilized powder is stable at room temperature but it is best stored desiccated below 0 Deg C. Reconstituted human MIP-3 should be stored in working aliquots at -20 DEG C. AVOID FREQUENT FREEZE THAW CYCLES. EACh FREEZE THAW CYCLE WILL DIMINISH ACTIVITY.

AA Sequence :



Reactivity: The biological activity of human MIP-3 was determined by a chemotaxis assay using total T cell population. Maxium chemotaxis was acheived using a concentration of 10ng/ml.

For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.  It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.


Return (human cytokine index)

RDI Divison of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049


phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

Ordering terms

all rights reserved by any/all copyright holders?