rev: 5/18/2008
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Human Cytokine/Growth Factor/Chemokines Sample Spec sheets (subject to change without notice):
DATA SHEET:recombinant Human FLT3-LIGAND
-Human FLT3-ligand (flk2-ligand) stimulates the proliferation and colony formation of certain BM precursor cells including CD34+ cells. This rec FLT3-ligand is a soluble 17.6kDa polypeptide containing 155 amino acid residues which comprises the extracellular domain of the transmembrane flt3-ligand protein.
Catalog#: RDI-3019
Package Size: 10 micrograms $258.50
Mini-Pack: cat#RDI-3019P $104.50/2ug
Supplied: Lyophilized powder with no additives.
Source: E. Coli
Purity: >98% by SDS-Page analysis. Endotoxin
level is less than 0.1ng per ug of flt3-ligand.
Reconstitution: The lyophilized flt-3 ligand should
be reconstituted in water or other aqueous buffers to a concentration of 100 nanograms/ul .
Allow to set for at least 30 minutes, tighten cap, rotate, and mix well. If desired, centrifuge for 1 minutes
at 1000 rpm to concentrate material in vial. This solution should be diluted
into buffer containing carrier protein or medium containing serum.
-Note:If this material is not to be used for coating
or labeling (or other applications requiring carrier free material), the
addition of 0.1% BSA to dilution buffers or dilution in sterile culture media
is recommended to enhance stability and minimize absorption to vial. Ideally,
maintain stock >50ug/ml.
Storage: The lyophilized powder is stable at room temperature but it is best
stored desiccated below 0 Deg C. reconstituted material should be stored in
working aliquots at -20 DEG C. AVOID FREQUENT FREEZE THAW CYCLES.
Reactivity: Reactivity: The ED50 as determined by the dose dependent
stimulation of the proliferation of human AML5 cells is <= 1.0ng/ml,
corresponding to a specific activity of > 1million units/mg.
AA Sequence:
MTQDCSFQHS PISSDFAVKI RELSDYLLQD YPVTVASNLQ
DEELCGGLWR LVLAQRWMER LKTVAGSKMQ GLLERVNTEI
HFVTKCAFQP PPSCLRFVQT NISRLLQETS EQLVALKPWI
TRQNFSRCLE LQCQPDSSTL PPPWSPRPLE ATAPTA
Precautions:For In vitro
research Use Only. Not for use in or on humans or animals or for diagnostics.
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