rev:    October 19, 2004

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Murine/Rat Cytokine/Growth Factor/Chemokines Sample Spec sheets (subject to change without notice):


DATA SHEET:recombinant Mouse FLT-3 LIGAND

-Flt3-Ligand belongs to a small group of growth factors that regulates proliferation of early hematopoietic cells. Multiple isoforms of Flt3-Ligand have been identified. The predominant form is the transmembrane which is biologically active on the cell surface. The proteolytically cleaved transmembrane isoform generates a soluble form, which is also biologically active. Flt3-Ligand binds to cells expressing the tyrosine kinase receptor Flt3. Alone, Flt3-Ligand cannot stimulate proliferation, but synergizes well with other CSFs and interleukins to induce growth and differentiation. Flt3-Ligand has no activity on mast cells. This recombinant Murine Flt3-Ligand is a 16.4 kDa protein containing 144 amino acid residues.

Catalog#: RDI-2531

Package Size:

10 micrograms $235.00   $219.00/package 2 or more (any combination)

                        -Mini-Pack# cat#RDI-2531p $94.00/2ug

                   -inquire for custom sizes/bulk packaging

Supplied: Lyophilized powder with no additives.

Source: E. Coli

Purity: >98% by SDS-Page and HPLC analysis. Endotoxin level is less than 0.1ng per ug (1EU/ug) of mouse FLT-3L

Reconstitution: At first use, tighten cap, genetly tap on counter or centrifuge 1 minute 1000 rpm to concentrate powder in bottom of vial. The lyophilzed FLT-3L should then be reconstituted in sterile DI water to a concentration of 0.1- 1mg/ml. Allow to set at least 30 minutes at 4 DEG C, Mix well. If desired, centrifuge for 1-2 minutes at 1000 rpm to concentrate material in vial. This solution can be diluted into other buffered solutions or stored at 4 DEG C for 1 week or -20 DEG C for future use.

Note:If this material is not to be used for coating or labeling (or other applications requiring carrier free material), the addition of 0.1% BSA to later dilution buffers or dilution in sterile culture media is recommended to enhance stability and minimize absorption to vial. Ideally, maintain stock >10ug/ml.

Storage: The lyophilized powder is stable at room temperature for a few weeks but it is best stored desiccated at - 20 DEG C. Reconstituted FLT-3 ligand should be stored in working aliquots at -20 DEG C. AVOID FREQUENT FREEZE THAW CYCLES.

Reactivity: Determined by its ability to generate CD11c+ splenic dendritic cells in vivo in C57B1/6 mice (females, approx. 12 weeks old). To obtain the desired affect, mice were treated with approximately 25ug of flt3-ligand once daily for 9 consecutive days via intraperitoneal injection.

AA Sequence:

MTPDCYFSHS PISSNFKVKF RQLTDHLLKD

YPVTVAVNLE DQKHCKALWS LFLAERWIQE

LKTVAGSKME TLLQDVNTQI HFVTSCTFEP

LPQCLRFVET NISHLLKDTC TELLALKPCI

GKACENFSRC LQVECEPGNG GPRA

Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.  It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.


see also human FLT-3L

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