Murine\RAT Cytokine Data Sheets (Mouse FLT3-Ligand) available from RDI Division of Fitzgerald Industries Intl

rev: October 19, 2004

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Murine/Rat Cytokine/Growth Factor/Chemokines Sample Spec sheets (subject to change without notice):

DATA SHEET:recombinant Mouse FLT-3 LIGAND

-Flt3-Ligand belongs to a small group of growth factors that regulates proliferation of early hematopoietic cells. Multiple isoforms of Flt3-Ligand have been identified. The predominant form is the transmembrane which is biologically active on the cell surface. The proteolytically cleaved transmembrane isoform generates a soluble form, which is also biologically active. Flt3-Ligand binds to cells expressing the tyrosine kinase receptor Flt3. Alone, Flt3-Ligand cannot stimulate proliferation, but synergizes well with other CSFs and interleukins to induce growth and differentiation. Flt3-Ligand has no activity on mast cells. This recombinant Murine Flt3-Ligand is a 16.4 kDa protein containing 144 amino acid residues.

Catalog#: RDI-2531

Package Size:

10 micrograms $235.00 $219.00/package 2 or more (any combination)

-Mini-Pack# cat#RDI-2531p $94.00/2ug

-inquire for custom sizes/bulk packaging

Supplied: Lyophilized powder with no additives.

Source: E. Coli

Purity: >98% by SDS-Page and HPLC analysis. Endotoxin level is less than 0.1ng per ug (1EU/ug) of mouse FLT-3L

Reconstitution: At first use, tighten cap, genetly tap on counter or centrifuge 1 minute 1000 rpm to concentrate powder in bottom of vial. The lyophilzed FLT-3L should then be reconstituted in sterile DI water to a concentration of 0.1- 1mg/ml. Allow to set at least 30 minutes at 4 DEG C, Mix well. If desired, centrifuge for 1-2 minutes at 1000 rpm to concentrate material in vial. This solution can be diluted into other buffered solutions or stored at 4 DEG C for 1 week or -20 DEG C for future use.

Note:If this material is not to be used for coating or labeling (or other applications requiring carrier free material), the addition of 0.1% BSA to later dilution buffers or dilution in sterile culture media is recommended to enhance stability and minimize absorption to vial. Ideally, maintain stock >10ug/ml.

Storage: The lyophilized powder is stable at room temperature for a few weeks but it is best stored desiccated at - 20 DEG C. Reconstituted FLT-3 ligand should be stored in working aliquots at -20 DEG C. AVOID FREQUENT FREEZE THAW CYCLES.

Reactivity: Determined by its ability to generate CD11c+ splenic dendritic cells in vivo in C57B1/6 mice (females, approx. 12 weeks old). To obtain the desired affect, mice were treated with approximately 25ug of flt3-ligand once daily for 9 consecutive days via intraperitoneal injection.

AA Sequence:

MTPDCYFSHS PISSNFKVKF RQLTDHLLKD

YPVTVAVNLE DQKHCKALWS LFLAERWIQE

LKTVAGSKME TLLQDVNTQI HFVTSCTFEP

LPQCLRFVET NISHLLKDTC TELLALKPCI

GKACENFSRC LQVECEPGNG GPRA

Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.