rev: October 19, 2004
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Murine/Rat Cytokine/Growth Factor/Chemokines Sample Spec sheets (subject to change without notice):
Mini-Pack:cat#RDI-2530P $94.00/5ug
-inquire for custom sizes/bulk packaging
Supplied: The sterile filtered solution was lyophilized from 10mM NaCitrate
pH4.0
Source: E. Coli
Purity: >98% by SDS-Page analysis Endotoxin <1EU/ug
Reconstitution: The lyophilized murine MEC should be reconstituted in water or 10mM NaCitrate pH4.0 to a concentration of 0.1-1mg/ml. Allow to set at least 30 minutes at 4-8 DEG C, Mix well. If desired, centrifuge for 1-2 minutes at 1000 rpm to concentrate material in vial.This solution can be diluted into other buffered solutions and stored at 4 DEG C for up to 1 week or stored at -20 DEG C for future use.
Note: If this material is not to be used for coating or labeling (or other applications requiring carrier free material), the addition of 0.1% BSA to dilution buffers or dilution in sterile culture media is recommended to enhance stability and minimize absorption to vial. Ideally, maintain stock >20ug/ml.
Storage: The lyophilized powder is stable at room temperature for a few weeks but it is best stored desiccated at -20 Deg C. Reconstituted material should be stored in working aliquots at -20DEG C. AVOID FREQUENT FREEZE THAW CYCLES.
Reactivity: Determined by its ability to chemoattract murine lymphocytes using a concentration range of 1.0 - 10.0 ng/ml.
Precautions: For In vitro research Use Only. Not for use in or on humans
or animals or for diagnostics. It is the responsibility of the user
to comply with all local/state and Federal rules in the use of this product.
We are not responsible for any patent infringements that might result
with the use of or derivation of this product.
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