rev: October 19, 2004
Return (RDI HOME PAGE)
Return (mouse cytokine index)
Murine/Rat Cytokine/Growth Factor/Chemokines Sample Spec sheets (subject to change without notice):
-Macrophage inflammatory protein- 3 beta (MIP-3 beta)also known as Exodus-3 and ELC, is a CC chemokine that is chemotactic towards T and B lymphocytes and myeloid progenitor cells but not towards monocytes and granulocytes. MIP-3 beta shares the CCR7 receptor with Exodus-2. It also plays an important role in the inflammatory response of human T cells and tissue macrophages. Recombinant Murine MIP-3 beta is a 9.2 kDa protein, containing 83 amino acid residues.
20 micrograms $231.00 $219.00/package 2 or more (any combination)
-Mini-Pack# cat#RDI-2527Bp $94.00/5ug -inquire for custom sizes/bulk packaging
Supplied: Lyophilized powder with no additives.
Source: E. Coli
Purity: >98% by SDS-Page and HPLC analysis. Endotoxin level is less than 0.1ng per ug (1EU/ug) of MIP-3 beta
Reconstitution: At first use, tighten cap, genetly tap on counter or centrifuge 1 minute 1000 rpm to concentrate powder in bottom of vial. The lyophilized MIP-3B should then be reconstituted isterile DI water to a concentration of 0.1- 1mg/ml. Allow to set at least 30 minutes at 4 DEG C, Mix well. If desired, centrifuge for 1-2 minutes at 1000 rpm to concentrate material in vial. This solution can be diluted into other buffered solutions or stored at 4 DEG C for 1 week or -20 DEG C for future use.
Note:If this material is not to be used for coating or labeling (or other applications requiring carrier free material), the addition of 0.1% BSA to later dilution buffers or dilution in sterile culture media is recommended to enhance stability and minimize absorption to vial. Ideally, maintain stock >10ug/ml.
Storage: The lyophilized powder is stable at room temperature for a few weeks but it is best stored desiccated at - 20 DEG C. Reconstituted MIP-3B should be stored in working aliquots at -20 DEG C. AVOID FREQUENT FREEZE THAW CYCLES.
Reactivity: Determined by its ability to chemoattract human mature dendritic cells using a concentration range of 10.0-100.0 ng/ml.
Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.
Return (RDI HOME PAGE)
RDI Divison of Fitzgerald Industries Intl
34 Junction Square Drive
Concord MA 01742-3049
phone (978) 371-6446 or (800) 370-2222
fax (978) 371-2266
all rights reserved by any/all copyright holders?