rev: October 19, 2004
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Murine/Rat Cytokine/Growth Factor/Chemokines Sample Spec
sheets (subject to change without notice):
DATA SHEET:recombinant MURINE Eotaxin-2
-Eotaxin-2 is a recently discovered CC chemokine characterized by its high
chemotactic selectivity for eosinophils. Recombinant Murine Eotaxin-2 is
a 10.1 kDa protein containing 91 amino acid residues.
Catalog#: RDI-2522
Package Size: 20 micrograms $231.00 $219.00/package 2+
mini-Pack: cat#RDI-2522P $94.00/2ug
-inquire for custom
sizes/bulk packaging
Supplied: Lyophilized powder with no additives.
Source: E. Coli
Purity: >98% by SDS-Page and HPLC analysis Endotoxin level is less than
0.1ng per ug (1EU/ug)
Reconstitution: The lyophilized eotaxin-2 should be reconstituted in water
to a concentration of 0.5-1.0mg/ml. Allow to set at least 30 minutes at 4
DEG C , tighten cap and rotate to reconstitute any material on sides of vial.
If desired, you can centrifuge at 1000rpm for 1 minute to concentrate volume
in bottom of vial. This solution can be diluted into water or other buffered
solutions containing carrier protein or medium containing serum.This solution
can be diluted into other buffered solutions or stored at -20 DEG C for future
use.
Note:If this material is not to be used for coating or labeling (or other
applications requiring carrier free material), the addition of 0.1% BSA or
dilution in sterile culture media is recommended to enhance stability and
minimize absorption to vial.
Storage: The lyophilized powder is stable at room temperature for a few weeks
but it is best stored desiccated at -20 DEG C. Reconstituted eotaxin-2 should
be stored in working aliquots at -20DEG C. AVOID FREQUENT FREEZE THAW
CYCLES.
Reactivity: Determined by its ability to chemoattract CCR3 transfected HEK/293
cells using a concentration range of 50-100 ng/ml.
AA Sequence: VTIPSSCCTS FISKKIPENR VVSYQLANGS ICPKAGVIFI
TKKGHKICTD PKLLWVQRHI QKLDAKKNQP SKGAKAVRTK
FAVQRRRGNS TEV
Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.
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RDI Division of Fitzgerald Industries Intl
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USA
phone (978) 371-6446 or (800) 370-2222
fax (978) 371-2266
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