rev: October 19, 2004
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Murine/Rat Cytokine/Growth Factor/Chemokines Sample Spec
sheets (subject to change without notice):
-Stromal Cell-Derived Factor-1 alpha (SDF1 alpha) is a recently discovered
protein belonging to the alpha chemokine (CXC) family of cytokines. THis
recombinant Murine SDF1 alpha is a 7.9 kDa protein containing 68 amino acid
residues.
Catalog#: RDI-2520A
Package Size: 10 micrograms $231.00 $219.00/package 2+
Mini-Pack: cat#RDI-2520AXP $94.00/2ug
-Bulk Quotes upon request -inquire for custom sizes/bulk packaging
Supplied: The sterile filtered solution was lyophilized from 10 mM Acetic
Acid, no additives/carrier
Source: E. Coli
Purity: >98% by SDS-Page and HPLC analysis Endotoxin level is less than
0.1ng per ug (1EU/ug) of SDF-1A
Reconstitution: The lyophilized SDF-1A should be reconstituted in water to
a concentration of 0.1- 1.0mg/ml. Allow to set at least 30 minutes at 4 DEG
C, tighten cap and rotate to reconstitute any material on sides of vial.
If desired, you can centrifuge at 1000 rpm for 1 minute to concentrate volume
in bottom of vial. This solution can be diluted into other buffered solutions
containing carrier protein or medium and stored at 4 DEg C for up to 1 week
or at -20 DEG C for future use.
Note:If this material is not to be used for coating or labeling (or other applications requiring carrier free material), the addition of 0.1% BSA to dilution buffers or dilution in sterile culture media is recommended to enhance stability and minimize absorption to vial. Ideally, maintain stock >10ug/ml.
Storage: The lyophilized powder is stable at room temperature for a few weeks but it is best stored desiccated at -20 DEg C. Reconstituted SDF-1A should be stored in working aliquots at -20DEG C. AVOID FREQUENT FREEZE THAW CYCLES.
Reactivity: determined by its ability to chemoattract human monocytes using a concentration range of 50- 100ng/ml.
Sequence:
KPVSLSYRCP CRFFESHIAR ANVKHLKILN TPNCALQIVA
RLKNNNRQVC IDPKLKWIQE YLEKALNK
Precautions:For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.
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